| Autor: |
Barone LJ; Institute of Virology and Technical Innovations, INTA- CONICET. National Research Council (CONICET), Buenos Aires, Argentina., Cardoso NP; Institute of Virology and Technical Innovations, INTA- CONICET. National Research Council (CONICET), Buenos Aires, Argentina., Mansilla FC; Institute of Virology and Technical Innovations, INTA- CONICET. National Research Council (CONICET), Buenos Aires, Argentina., Castillo M; Institute of Virology and Technical Innovations, INTA- CONICET. National Research Council (CONICET), Buenos Aires, Argentina., Capozzo AV; Institute of Virology and Technical Innovations, INTA- CONICET. National Research Council (CONICET), Buenos Aires, Argentina. |
| Abstrakt: |
Macrophages are important cells of the innate immunity that play a major role in Bovine Viral Diarrhea Virus (BVDV) pathogenesis. Macrophages are not a homogenous population; they exist in different phenotypes, typically divided into two main categories: classically (pro-inflammatory) and alternatively activated (anti-inflammatory) or M1 and M2, respectively. The role of bovine macrophage phenotypes on BVDV infection is still unclear. This study characterized the interaction between BVDV, and monocyte-derived macrophages (Mo-Mφ) collected from healthy cattle and polarized to an M1 or M2 state by using LPS, INF-γ, IL-4 or azithromycin. Arginase activity quantitation was utilized as a marker of the M2 Mo-Mφ spectrum. There was a significant association between arginase activity and the replication rate of BVDV strains of different genotypes and biotypes. Inhibition of arginase activity also reduced BVDV infectivity. Calves treated with azithromycin induced Mo-Mφ of the M2 state produced high levels of arginase. Interestingly, azithromycin administered in vivo increased the susceptibility of macrophages to BVDV infection ex vivo . Mo-Mφ from pregnant dams and calves produced higher arginase levels than those from non-pregnant adult animals. The increased infection of arginase-producing alternatively activated bovine macrophages with BVDV supports the need to delve into a possible leading role of M2 macrophages in establishing the immune-suppressive state during BVDV convalescence. |
