Propagation, Purification, and Characterization of Bacteriophages for Phage Therapy.
| Autor: | Kosznik-Kwaśnicka K; Department of Medical Microbiology, Faculty of Medicine, Medical University of Gdansk, Gdansk, Poland., Topka G; Univentum Labs Ltd., The UG SPV, Gdansk, Poland., Mantej J; Univentum Labs Ltd., The UG SPV, Gdansk, Poland., Grabowski Ł; Laboratory of Phage Therapy, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Gdansk, Poland., Necel A; Department of Medical Microbiology, Faculty of Medicine, Medical University of Gdansk, Gdansk, Poland., Węgrzyn G; Department of Molecular Biology, Faculty of Biology, University of Gdansk, Gdansk, Poland., Węgrzyn A; Phage Therapy Laboratory, University Center for Applied and Interdisciplinary Research, University of Gdansk, Gdansk, Poland. alicja.wegrzyn@ug.edu.pl. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2738, pp. 357-400. |
| DOI: | 10.1007/978-1-0716-3549-0_22 |
| Abstrakt: | Phage therapy is an alternative approach to combat bacterial infections. In this approach, bacteriophages are used as antimicrobial agents due to their properties to infect specific bacterial cells, to propagate inside their hosts, and to lyse host cell to release progeny phages. However, to introduce bacteriophages to clinical or veterinary practice, it is necessary to construct a large library of precisely characterized phages. Therefore, in this chapter, methods for propagation, purification, and microbiological characterization of bacteriophages are presented in the light of their potential use in phage therapy. Isolation of newly discovered bacteriophages from different habitats is also described as it is a preliminary assessment of their efficacy in combating bacterial biofilms and in the treatment of bacterial infections in a simple insect model-Galleria mellonella. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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