A laboratory framework for ongoing optimization of amplification-based genomic surveillance programs.

Autor: Lam C; Centre for Infectious Diseases and Microbiology - Public Health, Institute for Clinical Pathology and Medical Research Westmead Hospital , Westmead, Australia.; Faculty of Medicine and Health, Sydney Infectious Diseases Institute, The University of Sydney , Sydney, New South Wales, Australia., Johnson-Mackinnon J; Centre for Infectious Diseases and Microbiology - Public Health, Institute for Clinical Pathology and Medical Research Westmead Hospital , Westmead, Australia.; Faculty of Medicine and Health, Sydney Infectious Diseases Institute, The University of Sydney , Sydney, New South Wales, Australia., Basile K; Faculty of Medicine and Health, Sydney Infectious Diseases Institute, The University of Sydney , Sydney, New South Wales, Australia.; Centre for Infectious Diseases and Microbiology - Laboratory Services, Institute for Clinical Pathology and Medical Research, NSW Health Pathology , Sydney, Australia., Fong W; Centre for Infectious Diseases and Microbiology - Public Health, Institute for Clinical Pathology and Medical Research Westmead Hospital , Westmead, Australia.; Faculty of Medicine and Health, Sydney Infectious Diseases Institute, The University of Sydney , Sydney, New South Wales, Australia., Suster CJ; Centre for Infectious Diseases and Microbiology - Public Health, Institute for Clinical Pathology and Medical Research Westmead Hospital , Westmead, Australia.; Faculty of Medicine and Health, Sydney Infectious Diseases Institute, The University of Sydney , Sydney, New South Wales, Australia., Gall M; Centre for Infectious Diseases and Microbiology - Laboratory Services, Institute for Clinical Pathology and Medical Research, NSW Health Pathology , Sydney, Australia.; Faculty of Medicine and Health, School of Medical Sciences, The University of Sydney , Sydney, New South Wales, Australia., Agius J; Centre for Infectious Diseases and Microbiology - Public Health, Institute for Clinical Pathology and Medical Research Westmead Hospital , Westmead, Australia.; Faculty of Medicine and Health, Sydney Infectious Diseases Institute, The University of Sydney , Sydney, New South Wales, Australia., Chandra S; Centre for Infectious Diseases and Microbiology - Public Health, Institute for Clinical Pathology and Medical Research Westmead Hospital , Westmead, Australia.; Faculty of Medicine and Health, Sydney Infectious Diseases Institute, The University of Sydney , Sydney, New South Wales, Australia., Draper J; Centre for Infectious Diseases and Microbiology - Laboratory Services, Institute for Clinical Pathology and Medical Research, NSW Health Pathology , Sydney, Australia.; Faculty of Medicine and Health, School of Medical Sciences, The University of Sydney , Sydney, New South Wales, Australia., Martinez E; Centre for Infectious Diseases and Microbiology - Laboratory Services, Institute for Clinical Pathology and Medical Research, NSW Health Pathology , Sydney, Australia.; Faculty of Medicine and Health, School of Medical Sciences, The University of Sydney , Sydney, New South Wales, Australia., Drew A; Centre for Infectious Diseases and Microbiology - Laboratory Services, Institute for Clinical Pathology and Medical Research, NSW Health Pathology , Sydney, Australia.; Faculty of Medicine and Health, School of Medical Sciences, The University of Sydney , Sydney, New South Wales, Australia., Wang Q; Centre for Infectious Diseases and Microbiology - Laboratory Services, Institute for Clinical Pathology and Medical Research, NSW Health Pathology , Sydney, Australia.; Faculty of Medicine and Health, School of Medical Sciences, The University of Sydney , Sydney, New South Wales, Australia., Chen SC; Centre for Infectious Diseases and Microbiology - Laboratory Services, Institute for Clinical Pathology and Medical Research, NSW Health Pathology , Sydney, Australia.; Faculty of Medicine and Health, School of Medical Sciences, The University of Sydney , Sydney, New South Wales, Australia., Kok J; Centre for Infectious Diseases and Microbiology - Laboratory Services, Institute for Clinical Pathology and Medical Research, NSW Health Pathology , Sydney, Australia.; Faculty of Medicine and Health, School of Medical Sciences, The University of Sydney , Sydney, New South Wales, Australia., Dwyer DE; Centre for Infectious Diseases and Microbiology - Laboratory Services, Institute for Clinical Pathology and Medical Research, NSW Health Pathology , Sydney, Australia.; Faculty of Medicine and Health, School of Medical Sciences, The University of Sydney , Sydney, New South Wales, Australia., Sintchenko V; Centre for Infectious Diseases and Microbiology - Public Health, Institute for Clinical Pathology and Medical Research Westmead Hospital , Westmead, Australia.; Faculty of Medicine and Health, Sydney Infectious Diseases Institute, The University of Sydney , Sydney, New South Wales, Australia.; Faculty of Medicine and Health, School of Medical Sciences, The University of Sydney , Sydney, New South Wales, Australia., Rockett RJ; Centre for Infectious Diseases and Microbiology - Public Health, Institute for Clinical Pathology and Medical Research Westmead Hospital , Westmead, Australia.; Faculty of Medicine and Health, Sydney Infectious Diseases Institute, The University of Sydney , Sydney, New South Wales, Australia.; Faculty of Medicine and Health, School of Medical Sciences, The University of Sydney , Sydney, New South Wales, Australia.
Jazyk: angličtina
Zdroj: Microbiology spectrum [Microbiol Spectr] 2023 Dec 12; Vol. 11 (6), pp. e0220223. Date of Electronic Publication: 2023 Nov 15.
DOI: 10.1128/spectrum.02202-23
Abstrakt: Importance: This study provides a laboratory framework to ensure ongoing relevance and performance of amplification-based whole genome sequencing to strengthen public health surveillance during extended outbreaks or pandemics. The framework integrates regular reviews of the performance of a genomic surveillance system and highlights the importance of ongoing monitoring and the identification and implementation of improvements to whole genome sequencing methods to enhance public health responses to pathogen outbreaks.
Competing Interests: The authors declare no conflict of interest.
Databáze: MEDLINE