IgE response to Aed al 13 and Aed al 14 recombinant allergens from Aedes albopictus saliva in humans.

Autor:	Arnoldi I; Entopar Lab, Department of Biosciences, University of Milan, Via Celoria 26, 20133, Milan, Italy.; The Armenise-Harvard Laboratory of Structural Biology, Department Biology and Biotechnology, University of Pavia, Via Ferrata 9/A, 27100, Pavia, Italy.; Italian Malaria Network, Inter University Center for Malaria Research, University of Milan, Milan, 20133, Italy.; University School of Advanced Studies Pavia, IUSS, Pavia, 27100, Italy., Villa M; Entopar Lab, Department of Biosciences, University of Milan, Via Celoria 26, 20133, Milan, Italy., Mancini G; The Armenise-Harvard Laboratory of Structural Biology, Department Biology and Biotechnology, University of Pavia, Via Ferrata 9/A, 27100, Pavia, Italy.; University School of Advanced Studies Pavia, IUSS, Pavia, 27100, Italy., Varotto-Boccazzi I; Entopar Lab, Department of Biosciences, University of Milan, Via Celoria 26, 20133, Milan, Italy.; Italian Malaria Network, Inter University Center for Malaria Research, University of Milan, Milan, 20133, Italy., Yacoub MR; Unit of Immunology, Rheumatology, Allergy and Rare Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy., Asperti C; Unit of Immunology, Rheumatology, Allergy and Rare Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy., Mascheri A; Unit of Immunology, Rheumatology, Allergy and Rare Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy., Casiraghi S; Unit of Immunology, Rheumatology, Allergy and Rare Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy., Epis S; Entopar Lab, Department of Biosciences, University of Milan, Via Celoria 26, 20133, Milan, Italy.; Italian Malaria Network, Inter University Center for Malaria Research, University of Milan, Milan, 20133, Italy., Bandi C; Entopar Lab, Department of Biosciences, University of Milan, Via Celoria 26, 20133, Milan, Italy.; Italian Malaria Network, Inter University Center for Malaria Research, University of Milan, Milan, 20133, Italy., Dagna L; Unit of Immunology, Rheumatology, Allergy and Rare Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy., Forneris F; The Armenise-Harvard Laboratory of Structural Biology, Department Biology and Biotechnology, University of Pavia, Via Ferrata 9/A, 27100, Pavia, Italy., Gabrieli P; Entopar Lab, Department of Biosciences, University of Milan, Via Celoria 26, 20133, Milan, Italy.; Italian Malaria Network, Inter University Center for Malaria Research, University of Milan, Milan, 20133, Italy.
Jazyk:	angličtina
Zdroj:	The World Allergy Organization journal [World Allergy Organ J] 2023 Nov 02; Vol. 16 (11), pp. 100836. Date of Electronic Publication: 2023 Nov 02 (Print Publication: 2023).
DOI:	10.1016/j.waojou.2023.100836
Abstrakt:	Background: Mosquito bite is normally associated with mild allergic responses, but severe localized or systemic reactions are also possible. Reliable tools for the diagnosis of mosquito allergy are still unavailable. Here, we investigated the IgE response to 3 potential salivary allergens identified in the saliva of the tiger mosquito Aedes albopictus . Methods: Serum from 55 adult individuals (28 controls and 27 allergic people), were analysed using an in-house Enzyme Linked ImmunoSorbent Assay (ELISA) against the Salivary Gland Extract (SGE) and the recombinant proteins alb D7l2 (Aed al 2), alb Antigen5-3 (Aed al 13) and alb LIPS-2 (Aed al 14). Results: Fifteen of the 27 (56%) individuals having hypersensitive reactions to mosquito bites had IgE serum levels recognizing SGE. Negative sera did not show detectable levels of IgE targeting the SGE from the most common sympatric mosquito Culex pipiens. Among the positive individuals, 2 subjects displayed IgE targeting Aed al 2 (13%), while IgE recognizing Aed al 13 and Aed al 14 were detected in ten (67%) and seven (47%) individuals, respectively. Two sera from non-hypersensitive subjects had detectable levels of IgE targeting Aed al 13, suggesting possible cross-reaction with the homologue salivary proteins of multiple mosquito species or, more generally, of hematophagous insects. Conclusions: Our results indicate that Aed al 13 and Aed al 14 hold the potential to be developed as tools for the diagnosis of allergy to Ae. albopictus bites. Such tools would facilitate epidemiological studies on tiger mosquito allergy in humans and might foster the development of further protein-based assays to investigate cross-species allergies. Competing Interests: The authors report no competing interests. (© 2023 The Author(s).)
Databáze:	MEDLINE
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