Gene expression analyses reveal differences in children's response to malaria according to their age.

Autor:	Tebben K; University of Maryland, Baltimore., Yirampo S; Universite des Sciences des Techniques et des Technologies de Bamako., Coulibaly D; Universite des Sciences des Techniques et des Technologies de Bamako., Koné A; Universite des Sciences des Techniques et des Technologies de Bamako., Laurens M; University of Maryland, Baltimore., Stucke E; University of Maryland, Baltimore., Dembélé A; Universite des Sciences des Techniques et des Technologies de Bamako., Tolo Y; Universite des Sciences des Techniques et des Technologies de Bamako., Traoré K; Universite des Sciences des Techniques et des Technologies de Bamako., Niangaly A; Universite des Sciences des Techniques et des Technologies de Bamako., Berry A; University of Maryland, Baltimore., Kouriba B; Universite des Sciences des Techniques et des Technologies de Bamako., Plowe C; University of Maryland, Baltimore., Doumbo O; Universite des Sciences des Techniques et des Technologies de Bamako., Lyke K; University of Maryland., Takala-Harrison S; University of Maryland, Baltimore., Thera M; Malaria Research and Training Centre-International Center for Excellence in Research (MRTC-ICER)., Travassos M; University of Maryland, Baltimore., Serre D; University of Maryland, Baltimore.
Jazyk:	angličtina
Zdroj:	Research square [Res Sq] 2023 Oct 27. Date of Electronic Publication: 2023 Oct 27.
DOI:	10.21203/rs.3.rs-3487114/v1
Abstrakt:	In Bandiagara, Mali, children experience on average two clinical malaria episodes per season. However, even in the same transmission area, the number of uncomplicated symptomatic infections, and their parasitemia, vary dramatically among children. To examine the factors contributing to these variations, we simultaneously characterized the host and parasite gene expression profiles from 136 children with symptomatic falciparum malaria and analyzed the expression of 9,205 human and 2,484 Plasmodium genes. We used gene expression deconvolution to estimate the relative proportion of immune cells and parasite stages in each sample and to adjust the differential gene expression analyses. Parasitemia explained much of the variation in both host and parasite gene expression and revealed that infections with higher parasitemia had more neutrophils and fewer T cells, suggesting parasitemia-dependent neutrophil recruitment and/or T cell extravasation to secondary lymphoid organs. The child's age was also strongly correlated with gene expression variations. Plasmodium falciparum genes associated with age suggested that older children carried more male gametocytes, while host genes associated with age indicated a stronger innate response (through TLR and NLR signaling) in younger children and stronger adaptive immunity (through TCR and BCR signaling) in older children. These analyses highlight the variability in host responses and parasite regulation during P. falciparum symptomatic infections and emphasize the importance of considering the children's age when studying and treating malaria infections. Competing Interests: Competing interests: Authors declare that they have no competing interests.
Databáze:	MEDLINE
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