Lack of chromokinesin Klp-19 creates a more rigid midzone and affects force transmission during anaphase in C. elegans .

Autor: Zimyanin V; Department of Molecular Physiology and Biological Physics, University of Virginia, School of Medicine, Charlottesville, VA, USA.; Center for Membrane and Cell Physiology, University of Virginia School of Medicine, Charlottesville, VA, USA., Magaj M; Department of Molecular Physiology and Biological Physics, University of Virginia, School of Medicine, Charlottesville, VA, USA.; Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA, USA., Yu CH; Department of Electrical and Computer Engineering, University of California, Santa Barbara, CA, USA., Gibney T; Department of Biology, University of Virginia, Charlottesville, VA, USA., Mustafa B; Molecular and Cellular Biology and School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA., Horton X; Department of Molecular Physiology and Biological Physics, University of Virginia, School of Medicine, Charlottesville, VA, USA.; Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA, USA., Siller K; IT-Research Computing, University of Virginia, Charlottesville, VA, USA., Cueff L; CNRS, Univ Rennes, IGDR (Institut de Génétique et Dévelopement de Rennes) - UMR 6290, F-35000 Rennes, France., Bouvrais H; CNRS, Univ Rennes, IGDR (Institut de Génétique et Dévelopement de Rennes) - UMR 6290, F-35000 Rennes, France., Pécréaux J; CNRS, Univ Rennes, IGDR (Institut de Génétique et Dévelopement de Rennes) - UMR 6290, F-35000 Rennes, France., Needleman D; Molecular and Cellular Biology and School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA.; Center for Computational Biology, Flatiron Institute, New York, NY, USA., Redemann S; Department of Molecular Physiology and Biological Physics, University of Virginia, School of Medicine, Charlottesville, VA, USA.; Center for Membrane and Cell Physiology, University of Virginia School of Medicine, Charlottesville, VA, USA.; Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, VA, USA.
Jazyk: angličtina
Zdroj: BioRxiv : the preprint server for biology [bioRxiv] 2023 Oct 26. Date of Electronic Publication: 2023 Oct 26.
DOI: 10.1101/2023.10.26.564275
Abstrakt: Recent studies have highlighted the significance of the spindle midzone - the region positioned between chromosomes - in ensuring proper chromosome segregation. By combining advanced 3D electron tomography and cutting-edge light microscopy we have discovered a previously unknown role of the regulation of microtubule dynamics within the spindle midzone of C. elegans . Using Fluorescence recovery after photobleaching and a combination of second harmonic generation and two-photon fluorescence microscopy, we found that the length of the antiparallel microtubule overlap zone in the spindle midzone is constant throughout anaphase, and independent of cortical pulling forces as well as the presence of the microtubule bundling protein SPD-1. Further investigations of SPD-1 and the chromokinesin KLP-19 in C. elegans suggest that KLP-19 regulates the overlap length and functions independently of SPD-1. Our data shows that KLP-19 plays an active role in regulating the length and turn-over of microtubules within the midzone as well as the size of the antiparallel overlap region throughout mitosis. Depletion of KLP-19 in mitosis leads to an increase in microtubule length in the spindle midzone, which also leads to increased microtubule - microtubule interaction, thus building up a more robust microtubule network. The spindle is globally stiffer and more stable, which has implications for the transmission of forces within the spindle affecting chromosome segregation dynamics. Our data shows that by localizing KLP-19 to the spindle midzone in anaphase microtubule dynamics can be locally controlled allowing the formation of a functional midzone.
Databáze: MEDLINE
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