| Autor: |
Al-Rajhi AMH; Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh 11671, Saudi Arabia., Bakri MM; Biology Department, College of Science, Jazan University, Jazan 82817, Saudi Arabia., Qanash H; Department of Medical Laboratory Science, College of Applied Medical Sciences, University of Ha'il, Hail 55476, Saudi Arabia.; Medical and Diagnostic Research Center, University of Ha'il, Hail 55473, Saudi Arabia., Alzahrani HY; University Medical Service Center, King Abdulaziz University, Building 70, Jeddah 21589, Saudi Arabia., Halawani H; University Medical Service Center, King Abdulaziz University, Building 70, Jeddah 21589, Saudi Arabia., Algaydi MA; University Medical Service Center, King Abdulaziz University, Building 70, Jeddah 21589, Saudi Arabia., Abdelghany TM; Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11725, Egypt. |
| Jazyk: |
angličtina |
| Zdroj: |
Molecules (Basel, Switzerland) [Molecules] 2023 Nov 02; Vol. 28 (21). Date of Electronic Publication: 2023 Nov 02. |
| DOI: |
10.3390/molecules28217402 |
| Abstrakt: |
In the last decade, the urgent need to explore medicinal plants or drug development has increased enormously around the world to overcome numerous health problems. In the present investigation, HPLC indicated the existence of 18 phenolic and flavonoid compounds in the Cupressus sempervirens extract. Hesperetin represents the greatest concentration (25,579.57 µg/mL), while other compounds, such as pyro catechol, rutin, gallic acid, chlorogenic acid, naringenin, and quercetin, were recognized in concentrations of 2922.53 µg/mL, 1313.26 µg/mL, 1107.26 µg/mL, 389.09 µg/mL, 156.53 µg/mL, and 97.56 µg/mL, respectively. The well diffusion method documented the antibacterial/antifungal activity of C. sempervirens extract against E. faecalis , E. coli , C. albicans , S. typhi , S.aureus , and M. circinelloid with 35, 33, 32, 25, 23, and 21 mm inhibition zones, respectively, more than the standard antibiotic/antifungal agent. Low values ranging from 7.80 to 15.62 µg/mL of MIC and MBC were recorded for E. faecalis , E. coli , and C. albicans . From the 1- diphenyl-2-picryl hydrazyl (DPPH) assay, promising antioxidant activity was recorded for C. sempervirens extract with IC 50 of an 8.97 µg/mL. Moreover, ferric reducing antioxidant power (FRAP) and total antioxidant capacity assays (TAC) confirmed the antioxidant activity of the extract, which was expressed as the ascorbic acid equivalent (AAE) of 366.9 ± 0.2 µg/mg and 102 ± 0.2 µg/mg of extracts, respectively. α-amylase and α-glucosidase inhibition % were determined to express the antidiabetic activity of the extract in vitro, with promising IC 50 value (27.01 µg/mL) for α-amylase compared to that of acarbose (50.93 µg/mL), while IC 50 value of the extract for α-glucosidase was 19.21µg/mL compared to that of acarbose 4.13 µg/mL. Prothrombin time (PT) and activated partial thromboplastin time (APTT) revealed the role of C. sempervirens extract as an anticoagulant agent if compared with the activity of heparin. Binding interactions of hesperetin and gallic acid were examined via the Molecular Operating Environment (MOE) Dock software against E. faecalis (PDB ID: 3CLQ), C. albicans (PDB ID: 7RJC), α-amylase (PDB ID: 4W93), and α-glucosidase (PDB ID: 3TOP). The obtained results shed light on how molecular modeling methods might inhibit the tested compounds, which have the potential to be useful in the treatment of target proteins. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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