Structural Analysis and Characterization of an Antiproliferative Lectin from Canavalia villosa Seeds.

Autor: Lossio CF; Laboratory of Biologically Active Molecules, Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60440-970, Brazil., Osterne VJS; Laboratory of Biologically Active Molecules, Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60440-970, Brazil.; Laboratory of Biochemistry and Glycobiology, Department of Biotechnology, Ghent University, 9000 Ghent, Belgium., Pinto-Junior VR; Laboratory of Biologically Active Molecules, Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60440-970, Brazil.; Department of Physics, Federal University of Ceara, Fortaleza 60440-970, Brazil., Chen S; Laboratory of Biochemistry and Glycobiology, Department of Biotechnology, Ghent University, 9000 Ghent, Belgium., Oliveira MV; Laboratory of Biologically Active Molecules, Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60440-970, Brazil., Verduijn J; Nano-Biotechnology Group, Department of Biotechnology, Ghent University, 9000 Ghent, Belgium., Verbeke I; Laboratory of Biochemistry and Glycobiology, Department of Biotechnology, Ghent University, 9000 Ghent, Belgium., Serna S; Glycotechnology Lab, Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo de Miramon 194, 20014 Donostia-San Sebastián, Spain., Reichardt NC; Glycotechnology Lab, Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo de Miramon 194, 20014 Donostia-San Sebastián, Spain.; Centro de Investigación Biomédica en Red (CIBER-BBN), Paseo de Miramon 194, 20014 Donostia-San Sebastián, Spain., Skirtach A; Nano-Biotechnology Group, Department of Biotechnology, Ghent University, 9000 Ghent, Belgium., Cavada BS; Laboratory of Biologically Active Molecules, Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60440-970, Brazil., Van Damme EJM; Laboratory of Biochemistry and Glycobiology, Department of Biotechnology, Ghent University, 9000 Ghent, Belgium., Nascimento KS; Laboratory of Biologically Active Molecules, Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60440-970, Brazil.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2023 Nov 04; Vol. 24 (21). Date of Electronic Publication: 2023 Nov 04.
DOI: 10.3390/ijms242115966
Abstrakt: Cells use glycans to encode information that modulates processes ranging from cell-cell recognition to programmed cell death. This information is encoded within a glycocode, and its decoding is performed by carbohydrate-binding proteins. Among these, lectins stand out due to their specific and reversible interaction with carbohydrates. Changes in glycosylation patterns are observed in several pathologies, including cancer, where abnormal glycans are found on the surfaces of affected tissues. Given the importance of the bioprospection of promising biomolecules, the current work aimed to determine the structural properties and anticancer potential of the mannose-specific lectin from seeds of Canavalia villosa (Cvill). Experimental elucidation of the primary and 3D structures of the lectin, along with glycan array and molecular docking, facilitated the determination of its fine carbohydrate-binding specificity. These structural insights, coupled with the lectin's specificity, have been combined to explain the antiproliferative effect of Cvill against cancer cell lines. This effect is dependent on the carbohydrate-binding activity of Cvill and its uptake in the cells, with concomitant activation of autophagic and apoptotic pathways.
Databáze: MEDLINE
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