Antitumoral Potential of Artepillin C, a Compound Derived from Brazilian Propolis, against Breast Cancer Cell Lines.

Autor:	de Freitas Meirelles LE; Department of Clinical Analysis and Biomedicine, Universidade Estadual de Maringá (UEM), Maringá, Paraná, Brazil., de Assis Carvalho ARB; Department of Clinical Analysis and Biomedicine, Universidade Estadual de Maringá (UEM), Maringá, Paraná, Brazil., Ferreira Damke GMZ; Department of Clinical Analysis and Biomedicine, Universidade Estadual de Maringá (UEM), Maringá, Paraná, Brazil., Souza RP; Department of Clinical Analysis and Biomedicine, Universidade Estadual de Maringá (UEM), Maringá, Paraná, Brazil., Damke E; Department of Clinical Analysis and Biomedicine, Universidade Estadual de Maringá (UEM), Maringá, Paraná, Brazil., de Souza Bonfim-Mendonça P; Department of Clinical Analysis and Biomedicine, Universidade Estadual de Maringá (UEM), Maringá, Paraná, Brazil., de Oliveira Dembogurski DS; Laboratory of Natural Products and Mass Spectrometry (LAPNEM), Universidade Federal do Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil., da Silva DB; Laboratory of Natural Products and Mass Spectrometry (LAPNEM), Universidade Federal do Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil., Consolaro MEL; Department of Clinical Analysis and Biomedicine, Universidade Estadual de Maringá (UEM), Maringá, Paraná, Brazil., da Silva VRS; Department of Clinical Analysis and Biomedicine, Universidade Estadual de Maringá (UEM), Maringá, Paraná, Brazil.
Jazyk:	angličtina
Zdroj:	Anti-cancer agents in medicinal chemistry [Anticancer Agents Med Chem] 2024; Vol. 24 (2), pp. 117-124.
DOI:	10.2174/0118715206270534231103074433
Abstrakt:	Background: Breast cancer is the most commonly diagnosed cancer among women worldwide with limited treatment options. Artepillin C (3,5-diprenyl-4-hydroxycinnamic acid) is one of the main constituents of Brazilian propolis presenting different activities, including antitumoral effects against various types of cancer. Objective: We evaluated the antitumoral potential and mechanisms of action of artepillin C against two distinct human breast cancer cell lines, MCF-7 and MDA-MB-231, to explore a new therapeutic candidate. Methods: Cell viability was assessed by MTT assay and the long-term cytotoxicity was performed by clonogenic assay. The morphological changes were observed by light microscopy, analysis of cell death pathway by Annexin V FITC/propidium iodide (PI), lactate dehydrogenase (LDH) by colorimetry, DNA fragmentation by agarose gel and senescence by β-galactosidase. Detection of total reactive oxygen species (ROS) by fluorescence microscopy and determination of mitochondrial transmembrane potential by flow cytometry were also performed. Results: Artepillin C presented a strong and dose-time-dependent cytotoxic effect on MCF-7 and MDA-MB-231 cell lines, with cytotoxicity more evident in MCF-7. In both cancer cell lines, the clonogenic potential was significantly reduced and the morphology of the cells was changed. The treatment also induced death by necrosis and late apoptosis in MCF-7 and MDA-MB-231 and induced cell senescence in MCF-7. Also, artepillin C increased total ROS in both cancer cells and decreased mitochondrial membrane potential in MDA-MB-231 cells. Conclusion: Artepillin C presented antitumoral potential in two human breast cancer cell lines, MCF-7, and MDA-MB-231, suggesting a new promising option for the treatment and/or chemopreventive strategy for breast cancer. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
Databáze:	MEDLINE
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