Repeated Exposure to High-THC Cannabis Smoke during Gestation Alters Sex Ratio, Behavior, and Amygdala Gene Expression of Sprague Dawley Rat Offspring.

Autor:	Sandini TM; Department of Anatomy, Physiology, and Pharmacology, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada., Onofrychuk TJ; Department of Anatomy, Physiology, and Pharmacology, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada., Roebuck AJ; Department of Anatomy, Physiology, and Pharmacology, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada.; School of Liberal Arts, Yukon University, Whitehorse, Yukon Territory Y1A 5K4, Canada., Hammond SA; Global Institute for Food Security, Saskatoon, Saskatchewan S7N 4L8, Canada., Udenze D; Next Generation Sequencing Facility, Saskatoon, Saskatchewan S7N 5E5, Canada., Hayat S; Deparment of Oncology, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada., Herdzik MA; Department of Anatomy, Physiology, and Pharmacology, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada., McElroy DL; Department of Anatomy, Physiology, and Pharmacology, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada., Orvold SN; Department of Anatomy, Physiology, and Pharmacology, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada., Greba Q; Department of Anatomy, Physiology, and Pharmacology, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada., Laprairie RB; College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada john.howland@usask.ca robert.laprairie@usask.ca., Howland JG; Department of Anatomy, Physiology, and Pharmacology, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada john.howland@usask.ca robert.laprairie@usask.ca.
Jazyk:	angličtina
Zdroj:	ENeuro [eNeuro] 2023 Nov 28; Vol. 10 (11). Date of Electronic Publication: 2023 Nov 28 (Print Publication: 2023).
DOI:	10.1523/ENEURO.0100-23.2023
Abstrakt:	Because of the legalization of Cannabis in many jurisdictions and the trend of increasing Δ 9 -tetrahydrocannabinol (THC) content in Cannabis products, an urgent need exists to understand the impact of Cannabis use during pregnancy on fetal neurodevelopment and behavior. To this end, we exposed female Sprague Dawley rats to Cannabis smoke daily from gestational day 6 to 20 or room air. Maternal reproductive parameters, offspring behavior, and gene expression in the offspring amygdala were assessed. Body temperature was decreased in dams following smoke exposure and more fecal boli were observed in the chambers before and after smoke exposure in dams exposed to smoke. Maternal weight gain, food intake, gestational length, litter number, and litter weight were not altered by exposure to Cannabis smoke. A significant increase in the male-to-female ratio was noted in the Cannabis -exposed litters. In adulthood, male and female Cannabis smoke-exposed offspring explored the inner zone of an open field significantly less than control offspring. Gestational Cannabis smoke exposure did not affect behavior on the elevated plus maze test or social interaction test in the offspring. Cannabis offspring were better at visual pairwise discrimination and reversal learning tasks conducted in touchscreen-equipped operant conditioning chambers. Analysis of gene expression in the adult amygdala using RNA sequencing revealed subtle changes in genes related to development, cellular function, and nervous system disease in a subset of the male offspring. These results demonstrate that repeated exposure to high-THC Cannabis smoke during gestation alters maternal physiological parameters, sex ratio, and anxiety-like behaviors in the adulthood offspring. Competing Interests: R.B.L. is a member of the Scientific Advisory Board for Shackleford Pharma, which had no input into this research study. The authors declare no other competing financial interests. (Copyright © 2023 Sandini et al.)
Databáze:	MEDLINE
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