Microencapsulation of Bacillus thuringiensis strains for the control of Aedes aegypti.

Autor: Viana JL; Universidade do Estado do Amazonas - UEA, Programa de Pós-graduação em Biodiversidade e Biotecnologia da Rede BIONORTE - PPG BIONORTE, Av. Carvalho Leal, 1777, Ed. Anexo, 4° andar, Cachoeirinha, Manaus, CEP 69065001, AM, Brazil. Electronic address: julieteviana_2009@hotmail.com., da Silva JS; Curso Ciências Naturais, Campus VII, Universidade Federal do Maranhão, Avenida Dr. José Anselmo, 2008, São Sebastião, Codó, CEP 65400-000, MA, Brazil., de Mattos GC; Programa de Engenharia Química/COPPE - Universidade Federal do Rio de Janeiro, Cidade Universitária, Rio de Janeiro, CEP 21941-598, RJ, Brazil., Pinto MCC; Programa de Engenharia Química/COPPE - Universidade Federal do Rio de Janeiro, Cidade Universitária, Rio de Janeiro, CEP 21941-598, RJ, Brazil., Dutra LDS; Programa de Engenharia Química/COPPE - Universidade Federal do Rio de Janeiro, Cidade Universitária, Rio de Janeiro, CEP 21941-598, RJ, Brazil., Carvalho LLA; Programa de Engenharia Química/COPPE - Universidade Federal do Rio de Janeiro, Cidade Universitária, Rio de Janeiro, CEP 21941-598, RJ, Brazil., Pinto JCCDS; Programa de Engenharia Química/COPPE - Universidade Federal do Rio de Janeiro, Cidade Universitária, Rio de Janeiro, CEP 21941-598, RJ, Brazil., Pinheiro VCS; Laboratório de Entomologia Médica, Departamento de Química e Biologia, Universidade Estadual do Maranhão Campus Caxias, Praça Duque de Caxias, s/n, Morro do Alecrim, Caxias, CEP 65604-380, MA, Brazil., Roque RA; Instituto Nacional de Pesquisas da Amazônia, Laboratório de Controle Biológico e Biotecnologia da Malária e Dengue, Manaus, CEP 69060-001, AM, Brazil.
Jazyk: angličtina
Zdroj: Experimental parasitology [Exp Parasitol] 2023 Dec; Vol. 255, pp. 108654. Date of Electronic Publication: 2023 Nov 11.
DOI: 10.1016/j.exppara.2023.108654
Abstrakt: In this study, we investigated the microencapsulation of two strains of the entomopathogenic bacteria Bacillus thuringiensis (B. thuringiensis) (BtMA-750 and BtMA-1114), which are biopesticides of high toxicity for the mosquito vector Aedes aegypti. The encapsulation of different concentrations of microorganisms in starch microparticles was evaluated, and the inverse suspension polymerization technique was explored. It was possible to observe that the higher amounts of the biopesticide caused a slight decrease in the diameter of the particles; however, even when encapsulated, the biopesticide still presents an average diameter that is able to be consumed by the larvae of Aedes aegypti. Furthermore, it was noticed that the presence of both of the B. thuringiensis strains did not affect the thermal stability of the particles. The microencapsulated bacterial strains presented a high number of viable spores and preserved the expression of proteins with molecular masses corresponding to the insecticidal toxins Cry and Cyt, indicating that the encapsulation process was conducted satisfactorily. Finally, the encapsulated strains were tested against Ae. aegypti larvae and maintained 100% larval mortality even after 35 days. Therefore, microencapsulation of B. thuringiensis not only guarantees the bacterial activity, but also prolongs the action of the biopesticide. Collectively, such findings highlight the great potential of the new biopesticides, which may help to reduce the population indices of the mosquito vector Ae. aegypti via a sustainable and environment-friendly route.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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