A Fast HPLC/UV Method for Determination of Ketoprofen in Cellular Media.

Autor: Vozniuk O; BIOCEV, First Faculty of Medicine, Charles University, 252 20, Vestec, Czech Republic.; Department of Analytical Chemistry, Faculty of Chemical Engineering, University of Chemistry and Technology, 166 28, Prague, Czech Republic., Kejík Z; Department of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 455/2, 128 08, Prague, Czech Republic.; BIOCEV, First Faculty of Medicine, Charles University, 252 20, Vestec, Czech Republic.; Department of Analytical Chemistry, Faculty of Chemical Engineering, University of Chemistry and Technology, 166 28, Prague, Czech Republic., Veselá K; Department of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 455/2, 128 08, Prague, Czech Republic.; BIOCEV, First Faculty of Medicine, Charles University, 252 20, Vestec, Czech Republic., Skaličková M; Department of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 455/2, 128 08, Prague, Czech Republic.; BIOCEV, First Faculty of Medicine, Charles University, 252 20, Vestec, Czech Republic., Novotný P; Department of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 455/2, 128 08, Prague, Czech Republic.; BIOCEV, First Faculty of Medicine, Charles University, 252 20, Vestec, Czech Republic., Hromádka R; Department of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 455/2, 128 08, Prague, Czech Republic.; BIOCEV, First Faculty of Medicine, Charles University, 252 20, Vestec, Czech Republic., Hajduch J; Department of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 455/2, 128 08, Prague, Czech Republic.; BIOCEV, First Faculty of Medicine, Charles University, 252 20, Vestec, Czech Republic., Martásek P; Department of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 455/2, 128 08, Prague, Czech Republic., Jakubek M; Department of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 455/2, 128 08, Prague, Czech Republic.; BIOCEV, First Faculty of Medicine, Charles University, 252 20, Vestec, Czech Republic.; Department of Analytical Chemistry, Faculty of Chemical Engineering, University of Chemistry and Technology, 166 28, Prague, Czech Republic.
Jazyk: angličtina
Zdroj: ChemistryOpen [ChemistryOpen] 2024 Mar; Vol. 13 (3), pp. e202300147. Date of Electronic Publication: 2023 Nov 13.
DOI: 10.1002/open.202300147
Abstrakt: A simple, sensitive and quick HPLC method was developed for the determination of ketoprofen in cell culture media (EMEM, DMEM, RPMI). Separation was performed using a gradient on the C18 column with a mobile phase of acetonitrile and miliQ water acidified by 0.1 % (v/v) formic acid. The method was validated for parameters including linearity, accuracy, precision, limit of quantitation and limit of detection, as well as robustness. The response was found linear over the range of 3-100 μg/mL as demonstrated by the acquired value of correlation coefficient R2=0.9997. The described method is applicable for determination of various pharmacokinetic aspects of ketoprofen in vitro.
(© 2023 The Authors. ChemistryOpen published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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