| Autor: |
Ali W; Natural and Medical Sciences Research Centre, University of Nizwa, P O Box 33, PC 616, Birkat Almouz, Nizwa, Oman. usman.anwar@unizwa.edu.om., Jamshidi-Adegani F; Laboratory for Stem Cell & Regenerative Medicine, Natural and Medical Sciences Research Center, University of Nizwa, Nizwa, P. O. Box: 33, PC 616, Oman., Mirsanei Z; Laboratory for Stem Cell & Regenerative Medicine, Natural and Medical Sciences Research Center, University of Nizwa, Nizwa, P. O. Box: 33, PC 616, Oman.; Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Al-Kindi J; Laboratory for Stem Cell & Regenerative Medicine, Natural and Medical Sciences Research Center, University of Nizwa, Nizwa, P. O. Box: 33, PC 616, Oman., Vakilian S; Laboratory for Stem Cell & Regenerative Medicine, Natural and Medical Sciences Research Center, University of Nizwa, Nizwa, P. O. Box: 33, PC 616, Oman., Al-Broumi M; Natural and Medical Sciences Research Centre, University of Nizwa, P O Box 33, PC 616, Birkat Almouz, Nizwa, Oman. usman.anwar@unizwa.edu.om., Al-Hashmi S; Laboratory for Stem Cell & Regenerative Medicine, Natural and Medical Sciences Research Center, University of Nizwa, Nizwa, P. O. Box: 33, PC 616, Oman., Rawson JM; Department of Chemistry and Biochemistry, University of Windsor, 401 Sunset Ave, Windsor, ON, N9B3P4, Canada. jmrawson@uwindsor.ca., Al-Harrasi A; Natural and Medical Sciences Research Centre, University of Nizwa, P O Box 33, PC 616, Birkat Almouz, Nizwa, Oman. usman.anwar@unizwa.edu.om., Anwar MU; Natural and Medical Sciences Research Centre, University of Nizwa, P O Box 33, PC 616, Birkat Almouz, Nizwa, Oman. usman.anwar@unizwa.edu.om. |
| Abstrakt: |
A tridentate ligand LH3 ((2-hydroxy-3-methoxybenzylidene)-2-(hydroxyimino)propanehydrazide) comprising o -vanillin, hydrazone and oxime donor groups has been employed to prepare a series of tetranuclear Ln(III) complexes. The reaction of ligand LH3 with Ln(NO 3 ) 3 [Ln = Sm, Eu, Gd, Tb, Dy, Ho, Er] in MeOH yielded Ln 4 ( LH ) 6 (MeOH) 2 (Ln = Sm(1), Eu(2), Gd(3), Tb(4), Ho (6) and Er (7))] whereas the corresponding reaction with Dy(NO 3 ) 3 afforded Dy 4 ( LH ) 4 ( LH 2 ) 2 (OH) 2 (5). All complexes were characterized by various analytical techniques including single crystal X-ray diffraction, IR spectroscopy, UV-Vis spectroscopy, and elemental analysis. To investigate the potential of these lanthanide complexes for wound healing applications, their effects on fibroblast viability, migration, and M2 macrophage polarization were evaluated. The cytotoxicity assessment revealed that complexes 2(Eu), 4(Tb), 5(Dy), and 7(Er) significantly enhanced fibroblast viability compared to the negative control (NC). In vitro wound healing assay demonstrated that complexes 2(Eu) and 7(Eu) substantially promoted fibroblast migration compared to the NC. Moreover, complex 2(Eu) exhibited significant anti-inflammatory effects by reducing the phagocytic ability of lipopolysaccharide (LPS)-stimulated macrophage cells and attenuating nitric oxide (NO) production. In conclusion, among the series of complexes tested, complex 2(Eu) displayed the most potent anti-inflammatory effect on macrophage cells, while simultaneously promoting fibroblast viability and migration. This unique combination of properties renders complex 2 (Eu) highly promising for wound healing applications. |
