| Autor: |
Neto NAS; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Aguiar TKB; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Costa RJP; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Mesquita FP; Drug Research and Development Center, Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil., Oliveira LLB; Drug Research and Development Center, Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil., Moraes MEA; Drug Research and Development Center, Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil., Montenegro RC; Drug Research and Development Center, Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil., Carneiro RF; Department of Fisheries Engineering, Federal University of Ceará (UFC), Fortaleza, CE, Brazil., Nagano CS; Department of Fisheries Engineering, Federal University of Ceará (UFC), Fortaleza, CE, Brazil., Freitas CDT; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, Brazil., Souza PFN; Drug Research and Development Center, Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil. |
| Abstrakt: |
Staphylococcus aureus forms biofilms, a structure that protects bacterial cells, conferring more resistance to difficult treatment. Synthetic peptides surge as an alternative to overcome the biofilm of multidrug-resistant pathogens. Mo -CBP 3 -PepI, when combined with Ciprofloxacin, reduced preformed S. aureus biofilm by 50% at low concentrations (0.2 and 6.2 μg. mL -1 , respectively). The goal of this study was to evaluate the proteomic profile of biofilms after treatment with the Mo -CBP 3 -PepI combined with ciprofloxacin. Here, proteomic analysis confirmed with more depth previously described mechanisms and revealed changes in the accumulation of proteins related to DNA and protein metabolism, cell wall biosynthesis, redox metabolism, quorum sensing, and biofilm formation. Some proteins related to DNA and protein metabolism were reduced, while other proteins, like redox system proteins, disappeared in Ciprofloxacin+ Mo -CBP 3 -PepI treatment. Our results indicated a synergistic effect of these two molecules with several mechanisms against S. aureus biofilm and opened new doors for combined treatments with other drugs. |
