Puberty as a DOHaD programming window: high-fat diet induces long-term hepatic dysfunction in male rats.

Autor:	Dos Santos BG; Department of Physiological Sciences, Laboratory of Endocrine Physiology and Metabolism, Federal University of Goiás, Goiânia, GO, Brazil., Miranda RA; Department of Physiological Sciences, Laboratory of Endocrine Physiology, Institute of Biology Roberto Alcantara Gomes, Rio de Janeiro State University, Rio de Janeiro, Brazil., Saavedra LPJ; Department of Biotechnology, Genetics and Cell Biology, Laboratory of Secretion Cell Biology, State University of Maringá, Maringá, Brazil., Francisco FA; Department of Biotechnology, Genetics and Cell Biology, Laboratory of Secretion Cell Biology, State University of Maringá, Maringá, Brazil., Ribeiro MVG; Department of Biotechnology, Genetics and Cell Biology, Laboratory of Secretion Cell Biology, State University of Maringá, Maringá, Brazil., Oliveira Ferreira AR; Department of Biotechnology, Genetics and Cell Biology, Laboratory of Secretion Cell Biology, State University of Maringá, Maringá, Brazil., Ferreira-Junior MD; Department of Physiological Sciences, Laboratory of Endocrine Physiology and Metabolism, Federal University of Goiás, Goiânia, GO, Brazil., Cavalcante KVN; Department of Physiological Sciences, Laboratory of Endocrine Physiology and Metabolism, Federal University of Goiás, Goiânia, GO, Brazil., Xavier CH; Department of Physiological Sciences, Federal University of Goiás, Goiânia, GO, Brazil., de Moura EG; Department of Physiological Sciences, Laboratory of Endocrine Physiology, Institute of Biology Roberto Alcantara Gomes, Rio de Janeiro State University, Rio de Janeiro, Brazil., Lisboa PC; Department of Physiological Sciences, Laboratory of Endocrine Physiology, Institute of Biology Roberto Alcantara Gomes, Rio de Janeiro State University, Rio de Janeiro, Brazil., Mota APCD; Department of Physiological Sciences, Laboratory of Endocrine Physiology and Metabolism, Federal University of Goiás, Goiânia, GO, Brazil., Pedrino GR; Department of Physiological Sciences, Federal University of Goiás, Goiânia, GO, Brazil., Armitage JA; School of Medicine and IMPACT Institute, Deakin University, Waurn Ponds, Australia., Mathias PCF; Department of Biotechnology, Genetics and Cell Biology, Laboratory of Secretion Cell Biology, State University of Maringá, Maringá, Brazil., Palma-Rigo K; Department of Biotechnology, Genetics and Cell Biology, Laboratory of Secretion Cell Biology, State University of Maringá, Maringá, Brazil., Gomes RM; Department of Physiological Sciences, Laboratory of Endocrine Physiology and Metabolism, Federal University of Goiás, Goiânia, GO, Brazil.
Jazyk:	angličtina
Zdroj:	Journal of developmental origins of health and disease [J Dev Orig Health Dis] 2023 Oct; Vol. 14 (5), pp. 614-622. Date of Electronic Publication: 2023 Nov 13.
DOI:	10.1017/S2040174423000272
Abstrakt:	The aim of this study was to evaluate whether high-fat (HF) diet intake during puberty can program obesity as well as generate glucose imbalance and hepatic metabolic dysfunctions in adult life. Male Wistar rats were randomly assigned into two groups: rats fed standard chow (NF) and rats fed a HF from postnatal 30-day-old (PND30) until PND60. Then, both groups were fed a standard chow from PND60 until PND120. Euthanasia and samples collections occurred at PND120. HF animals were overweight (+11%) and had increased adiposity, hyperphagia (+12%), hyperglycaemia (+13%), hyperinsulinemia (+69%), and hypertriglyceridemia (+34%). Plasma glucose levels during intravenous glucose tolerance test (ivGTT) and intraperitoneal insulin tolerance test (ipITT) were also higher in the HF group, whereas K itt was significantly lower (-34%), suggesting reduced insulin sensitivity. In the same sense, HF animals present pancreatic islets hypertrophy and high β-cell mass. HF animals also had a significant increase in blood glucose levels during pyruvate tolerance test, indicating increased gluconeogenesis. Hepatic morphology analyses showed an increase in lipid inclusion in the HF group. Moreover, PEPCK and FAS protein expression were higher in the livers of the HF animals (+79% and + 37%, respectively). In conclusion, HF during puberty causes obese phenotype leading to glucose dyshomeostasis and nonalcoholic fatty liver disease, which can be related to the overexpression of proteins PEPCK and FAS.
Databáze:	MEDLINE
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