Improvement of bacterial blight resistance of the high yielding, fine-grain, rice variety, Gangavati sona through marker-assisted backcross breeding.

Autor: Manoj CA; University of Agricultural Sciences, Raichur, India.; ICAR-Indian Institute of Rice Research, Hyderabad, India., Muralidhara B; University of Agricultural Sciences, Raichur, India.; ICAR-Indian Institute of Rice Research, Hyderabad, India., Basavaraj PS; ICAR-Indian Institute of Rice Research, Hyderabad, India.; ICAR-National Institute of Abiotic Stress Management, Baramati, India., Honnappa M; University of Agricultural Sciences, Raichur, India.; ICAR-Indian Institute of Rice Research, Hyderabad, India., Ajitha V; ICAR-Indian Institute of Rice Research, Hyderabad, India., Aleena D; ICAR-Indian Institute of Rice Research, Hyderabad, India., Ishwaryalakshmi VG; ICAR-Indian Institute of Rice Research, Hyderabad, India., Usha G; ICAR-Indian Institute of Rice Research, Hyderabad, India., Gireesh C; ICAR-Indian Institute of Rice Research, Hyderabad, India., Senguttuvel P; ICAR-Indian Institute of Rice Research, Hyderabad, India., Kemparaju KB; ICAR-Indian Institute of Rice Research, Hyderabad, India., Rao LVS; ICAR-Indian Institute of Rice Research, Hyderabad, India., Basavaraj K; ICAR-Indian Institute of Rice Research, Hyderabad, India., Laha GS; ICAR-Indian Institute of Rice Research, Hyderabad, India., Sundaram RM; ICAR-Indian Institute of Rice Research, Hyderabad, India., Kumar R; ICAR-Indian Institute of Rice Research, Hyderabad, India., Rathod S; ICAR-Indian Institute of Rice Research, Hyderabad, India., Salimath PM; University of Agricultural Sciences, Raichur, India., Lokesha R; University of Agricultural Sciences, Raichur, India., Diwan J; University of Agricultural Sciences, Raichur, India., Nidagundi JM; University of Agricultural Sciences, Raichur, India., Gowrisankar M; Annamalai University, Chidambaram, Tamil Nadu India., Anantha MS; ICAR-Indian Institute of Rice Research, Hyderabad, India.
Jazyk: angličtina
Zdroj: 3 Biotech [3 Biotech] 2023 Dec; Vol. 13 (12), pp. 393. Date of Electronic Publication: 2023 Nov 10.
DOI: 10.1007/s13205-023-03828-0
Abstrakt: Gangavati sona (GS) is a high-yielding, fine-grain rice variety widely grown in the Tungabhadra command area in Karnataka, India; however, it is susceptible to bacterial blight (BB). Therefore, the present study was conducted to improve the GS variety for BB resistance. Three BB-resistant genes ( xa5, xa13, and Xa21 ) were introgressed into the genetic background of susceptible cultivar GS through marker-assisted backcrossing (MABB) by using Improved samba Mahsuri (ISM), a popular, high-yielding, bacterial blight resistant rice variety as a donor parent. Foreground selection was carried out using gene-specific markers, viz., xa5FM ( xa5 ), xa13prom ( xa13 ), and pTA248 ( Xa21 ), while background selection was carried out using well-distributed 64 polymorphic microsatellite markers. The true heterozygote F 1 was used as the male parent for backcrossing with GS to obtain BC 1 F 1 . The process was repeated in BC 1 F 1 generation, and a BC 2 F 1 plant (IGS-5-11-5) possessing all three target genes along with maximum recurrent parent genome (RPG) recovery (86.7%) was selfed to obtain BC 2 F 2 s. At BC 2 F 2 , a single triple gene homozygote plant (IGS-5-11-5-33) with 92.6% RPG recovery was identified and advanced to BC 2 F 5 by a pedigree method. At BC 2 F 5 , the seven best entries were selected, possessing all three resistance genes with high resistance levels against bacterial blight, yield level, and grain quality features equivalent to better than GS. The improved versions of GS will immensely benefit the farmers whose fields are endemic to BB.
Competing Interests: Conflict of interestThe authors declare that they have no competing interests.
(© King Abdulaziz City for Science and Technology 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)
Databáze: MEDLINE
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