SNP-based high-resolution typing of Chlamydia psittaci from humans and wild birds in Sweden: circulation of the Mat116 genotype reveals the transmission mode to humans.

Autor: Herrmann B; Department of Clinical Microbiology, Uppsala University Hospital, SE-751 85 Uppsala, Sweden; Department of Medical Sciences, Clinical Microbiology, Uppsala University, SE-751 85 Uppsala, Sweden. Electronic address: bjorn.herrmann@medsci.uu.se., Aaziz R; Anses, Laboratory for Animal Health, Bacterial Zoonosis Unit, Paris-Est University, F-94706 Maisons-Alfort, France., Kaden R; Department of Clinical Microbiology, Uppsala University Hospital, SE-751 85 Uppsala, Sweden; Science for Life Laboratory, Clinical Genomics Uppsala, SE-751 85 Uppsala, Sweden., Riedel HM; Department of Clinical Microbiology, Uppsala University Hospital, SE-751 85 Uppsala, Sweden; Department of Medical Sciences, Clinical Microbiology, Uppsala University, SE-751 85 Uppsala, Sweden., Spörndly-Nees E; Department of Pathology and Wildlife Diseases, National Veterinary Institute (SVA), SE-751 89 Uppsala, Sweden., Sandelin LL; Department of Communicable Diseases and Disease Control, Region Kalmar County, SE- 391 26 Kalmar, Sweden; Department of Biology and Environmental Science, Linnaeus University, SE-391 82 Kalmar, Sweden., Laroucau K; Anses, Laboratory for Animal Health, Bacterial Zoonosis Unit, Paris-Est University, F-94706 Maisons-Alfort, France.
Jazyk: angličtina
Zdroj: Microbes and infection [Microbes Infect] 2024 Mar-Apr; Vol. 26 (3), pp. 105251. Date of Electronic Publication: 2023 Nov 11.
DOI: 10.1016/j.micinf.2023.105251
Abstrakt: The incidence of Chlamydia psittaci respiratory tract infections in humans has increased in Sweden in recent years. This study aimed to identify the transmission route by genotyping C. psittaci from infected humans and birds. 42 human C. psittaci samples and 5 samples from C. psittaci-infected birds were collected. Genotyping was performed using ompA sequencing, Multi-locus sequence typing, and/or SNP-based high-resolution melting-PCR. Epidemiological data was also collected, and a phylogenetic analysis was conducted. Analysis of ompA provided limited resolution, while the SNP-based PCR analysis successfully detected the Mat116 genotype in 3/5 passerine birds and in 26/29 human cases, indicating a high prevalence of this genotype in the human population. These cases were associated with contact with wild birds, mainly through bird feeding during winter or other outdoor exposure. Human cases caused by other genotypes (psittacine and pigeon) were less common and were linked to exposure to caged birds or pigeons. The SNP-genotype Mat116 is rare, but predominated in this study. The use of SNP-based PCR provided a better understanding of the C. psittaci transmission from birds to humans compared to ompA analysis. In Sweden, human psittacosis appears mainly to be transmitted from garden birds during bird feeding in the winter season.
(Copyright © 2023 The Author(s). Published by Elsevier Masson SAS.. All rights reserved.)
Databáze: MEDLINE
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