Microbiome diversity and composition in Bemisia tabaci SSA1-SG1 whitefly are influenced by their host's life stage.

Autor: El Hamss H; Natural Resources Institute, University of Greenwich, Central Avenue, Chatham Maritime, Kent, United Kingdom. Electronic address: Hajar.elhamss@gmail.com., Maruthi MN; Natural Resources Institute, University of Greenwich, Central Avenue, Chatham Maritime, Kent, United Kingdom. Electronic address: m.n.maruthi@greenwich.ac.uk., Omongo CA; Root Crops Programme, National Crops Resource Research Institute (RCP-NaCRRI), Kampala, Uganda., Wang HL; College of Forestry, Hebei Agricultural University, Hebei, China., van Brunschot S; Natural Resources Institute, University of Greenwich, Central Avenue, Chatham Maritime, Kent, United Kingdom; School of Biological Sciences, The University of Queensland, Brisbane, QLD, Australia., Colvin J; Natural Resources Institute, University of Greenwich, Central Avenue, Chatham Maritime, Kent, United Kingdom., Delatte H; CIRAD, UMR PVBMT, Saint Pierre, France.
Jazyk: angličtina
Zdroj: Microbiological research [Microbiol Res] 2024 Jan; Vol. 278, pp. 127538. Date of Electronic Publication: 2023 Nov 03.
DOI: 10.1016/j.micres.2023.127538
Abstrakt: Within the Bemisia tabaci group of cryptic whitefly species, many are damaging agricultural pests and plant-virus vectors, conferring upon this group the status of one of the world's top 100 most invasive and destructive species, affecting farmers' income and threatening their livelihoods. Studies on the microbiome of whitefly life stages are scarce, although their composition and diversity greatly influence whitefly fitness and development. We used high-throughput sequencing to understand microbiome diversity in different developmental stages of the B. tabaci sub-Saharan Africa 1 (SSA1-SG1) species of the whitefly from Uganda. Endosymbionts (Portiera, Arsenophonus, Wolbachia, and Hemipteriphilus were detected but excluded from further statistical analysis as they were not influenced by life stage using Permutational Multivariate Analysis of Variance Using Distance Matrices (ADONIS, p = 0.925 and Bray, p = 0.903). Our results showed significant differences in the meta microbiome composition in different life stages of SSA1-SG1. The diversity was significantly higher in eggs (Shannon, p = 0.024; Simpson, p = 0.047) than that in nymphs and pupae, while the number of microbial species observed by the amplicon sequence variant (ASV) was not significant (n(ASV), p = 0.094). At the phylum and genus levels, the dominant constituents in the microbiome changed significantly during various developmental stages, with Halomonas being present in eggs, whereas Bacillus and Caldalkalibacillus were consistently found across all life stages. These findings provide the first description of differing meta microbiome diversity in the life stage of whiteflies, suggesting their putative role in whitefly development.
Competing Interests: Declaration of Competing Interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 The Authors. Published by Elsevier GmbH.. All rights reserved.)
Databáze: MEDLINE
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