Molecular genetic divergence analysis amongst high curcumin lines of Golden Crop (Curcuma longa L.) using SSR marker and use in trait-specific breeding.

Autor: Gogoi A; Academy of Scientific and Industrial Research, Ghaziabad, UP, 201002, India.; CSIR-North East Institute of Science and Technology, Jorhat, Assam, 785006, India., Munda S; CSIR-North East Institute of Science and Technology, Jorhat, Assam, 785006, India., Paw M; CSIR-North East Institute of Science and Technology, Jorhat, Assam, 785006, India., Begum T; CSIR-North East Institute of Science and Technology, Jorhat, Assam, 785006, India. twahira.begum24@gmail.com., Siddiqui MH; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia., Gaafar AZ; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia., Kesawat MS; Institute for Molecular Biology and Genetics, School of Biological Sciences, Seoul National University, Seoul, 08826, South Korea., Lal M; Academy of Scientific and Industrial Research, Ghaziabad, UP, 201002, India. drmohanlal80@gmail.com.; CSIR-North East Institute of Science and Technology, Jorhat, Assam, 785006, India. drmohanlal80@gmail.com.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Nov 11; Vol. 13 (1), pp. 19690. Date of Electronic Publication: 2023 Nov 11.
DOI: 10.1038/s41598-023-46779-5
Abstrakt: Curcuma longa L., is recognized worldwide as a medicinally and economically important plant species due to its curcumin content which is an industrially important compound. In this study, a total of 329 accessions were collected from four states of India and planted in the experimental farm of CSIR-NEIST, Jorhat, India, in augmented design. Among these, 152 high curcumin (> 1.50%) accessions were screened for molecular divergence study using 39 SSR primers. The primers showed the most efficient outcome with 2-8 allele/ loci and a total 163 number of alleles with 100% polymorphism. Cluster analysis revealed the construction of three clusters, out of which one cluster was geographically dependent, and germplasm was particularly from Assam state. Jaccard's pairwise coefficient showed maximum genetic dissimilarity of (0.75) between accession RRLJCL 3 and RRLJCL 126, indicating high variation as it was from two different states viz Arunachal Pradesh and Nagaland respectively and minimum genetic dissimilarity of (0.09) between RRLJCL 58 and RRLJCL 59 indicating significantly less variation as the two accessions were from same state, i.e., Arunachal Pradesh. Analysis of Molecular Variance (AMOVA) revealed high molecular variation within the population (87%) and significantly less variation among the population (13%). Additionally, Neighbour Joining dendrogram, Principal Component Analysis (PCA), and bar plot structure revealed similar clustering of germplasm. This diversity assessment will help in selecting the trait-specific genotypes, crop improvement program, conservation of gene pool, marker-assisted breeding, and quantitative trait loci identification. Moreover, to the best of our knowledge, it is the first molecular diversity report among 152 high curcumin lines of C. longa from North East India using 39 SSR primers.
(© 2023. The Author(s).)
Databáze: MEDLINE
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