Epigenetic Regulation of Ameloblast Differentiation by HMGN Proteins.

Autor:	He B; Protein Section, Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.; Craniofacial Anomalies and Regeneration Section, National Institute of Dental and Craniofacial Research, Bethesda, MD, USA., Kram V; Molecular Biology of Bones & Teeth Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA., Furusawa T; Protein Section, Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA., Duverger O; Craniofacial Anomalies and Regeneration Section, National Institute of Dental and Craniofacial Research, Bethesda, MD, USA., Chu EY; Department of General Dentistry, Operative Division, University of Maryland, School of Dentistry, Baltimore, MD, USA., Nanduri R; Protein Section, Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA., Ishikawa M; Department of Pathology and Laboratory Medicine and Department of Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA., Zhang P; Molecular Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA., Amendt BA; Department of Anatomy and Cell Biology, and the Craniofacial Anomalies Research Center, Carver College of Medicine, the University of Iowa, Iowa City, IA, USA., Lee JS; Craniofacial Anomalies and Regeneration Section, National Institute of Dental and Craniofacial Research, Bethesda, MD, USA., Bustin M; Protein Section, Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Jazyk:	angličtina
Zdroj:	Journal of dental research [J Dent Res] 2024 Jan; Vol. 103 (1), pp. 51-61. Date of Electronic Publication: 2023 Nov 10.
DOI:	10.1177/00220345231202468
Abstrakt:	Dental enamel formation is coordinated by ameloblast differentiation, production of enamel matrix proteins, and crystal growth. The factors regulating ameloblast differentiation are not fully understood. Here we show that the high mobility group N (HMGN) nucleosomal binding proteins modulate the rate of ameloblast differentiation and enamel formation. We found that HMGN1 and HMGN2 proteins are downregulated during mouse ameloblast differentiation. Genetically altered mice lacking HMGN1 and HMGN2 proteins show faster ameloblast differentiation and a higher rate of enamel deposition in mice molars and incisors. In vitro differentiation of induced pluripotent stem cells to dental epithelium cells showed that HMGN proteins modulate the expression and chromatin accessibility of ameloblast-specific genes and affect the binding of transcription factors epiprofin and PITX2 to ameloblast-specific genes. Our results suggest that HMGN proteins regulate ameloblast differentiation and enamel mineralization by modulating lineage-specific chromatin accessibility and transcription factor binding to ameloblast regulatory sites. Competing Interests: Declaration of Conflicting InterestsThe authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
Databáze:	MEDLINE
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