Construction and biological characterization of a cDNA infectious clone of wheat umbra-like virus in wheat and Nicotiana benthamiana.

Autor: Nouri S; Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506, USA. Electronic address: shahidehnr@ksu.edu., Zarzyńska-Nowak A; Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506, USA., Prakash V; Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506, USA.
Jazyk: angličtina
Zdroj: Virology [Virology] 2024 Jan; Vol. 589, pp. 109929. Date of Electronic Publication: 2023 Nov 03.
DOI: 10.1016/j.virol.2023.109929
Abstrakt: Umbravirus-like associated RNAs (ulaRNAs) are a new group of subviral RNAs associated with plants. Little is known about the biology of ulaRNAs. We recently reported wheat umbra-like virus (WULV) from Kansas fields. In this work, we generated a full-length cDNA clone of WULV which systemically infected N. benthamiana. While agroinfiltrated leaves demonstrated severe necrosis, upper leaves were symptomless. We also showed that WULV is capable of infecting wheat in the absence of a helper virus. Furthermore, and through sap inoculation, we demonstrated that WULV is transmissible in the form of free RNA. This is the first report of a mechanically transmissible ulaRNA. Together, these findings contribute to advancing our knowledge of the biology of WULV. Moreover, the construction of the WULV infectious clone provides a valuable research tool for further investigations including the role of WULV in symptom development in interaction with other wheat viruses.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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