Transcriptome and metabolome analysis reveals stage-specific metabolite accumulation during maturity of Chinese black truffle Tuber indicum.

Autor: Li Y; Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. Electronic address: 15114605567@163.com., Xing YM; Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. Electronic address: meimary@163.com., Murat C; Université de Lorraine, INRAE, UMR Interactions Arbres/Microorganismes, INRAE Grand Est - Nancy, Champenoux, France. Electronic address: claude.murat@inrae.fr., Kohler A; Université de Lorraine, INRAE, UMR Interactions Arbres/Microorganismes, INRAE Grand Est - Nancy, Champenoux, France. Electronic address: annegret.kohler@inrae.fr., Zhou DY; Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. Electronic address: 3488427294@qq.com., Yu FQ; Key Laboratory for Fungal Diversity and Green Development, The Germplasm Bank of Wild Species, Kunming, Kunming Institute Botany, Chinese Academy of Sciences, Yunnan, China. Electronic address: fqyu@mail.kib.ac.cn., Chen J; Key Laboratory of Bioactive Substances and Resource Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. Electronic address: kibchenjuan@126.com.
Jazyk: angličtina
Zdroj: Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2023 Dec; Vol. 205, pp. 108158. Date of Electronic Publication: 2023 Nov 02.
DOI: 10.1016/j.plaphy.2023.108158
Abstrakt: Tuber indicum is the most economically important member of Tuber, with the highest production and widest distribution in China. However, the overexploitation of immature ascocarps not only has driven wild resources of the species toward extinction, but also has caused enconomic losses and a decline in the reputation of T.indicum quality. In this study, stage-specific metabolites of T. indicum in relation to nutritional quality and the mechanism of their accumulations were explored by transcriptome and metabolome analysis at five harvest times, representing four maturation stages. A total of 663 compounds were identified in T. indicum ascocarps by a widely targeted metabolomic approach. Lipid compounds are the most prominent metabolites (18%) in our samples and also are higher accumulation at the immature stage than at mature stage, representing 30.16% differential accumulated metabolites in this stage. Levels of some of the amino acids, such as S-(methyl) glutathione, S-adenosylmethionine, which are known truffle aroma precursors, were increased at the mature stage. The gene expression level related to the biosynthesis of volatile organic compounds were verified by qPCR. This study contributes to the preliminary understanding of metabolites variations in T. indicum ascocarps during maturity for quality evaluation and truffle biology.
Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest.
(Copyright © 2023 Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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