Protocol for tissue processing and paraffin embedding of mouse brains following ex vivo MRI.

Autor:	Smart A; Neuropathology, Nuffield Department of Clinical Neurosciences, University of Oxford, OX3 9DU Oxford, UK; Wellcome Centre for Integrative Neuroimaging, FMRIB, Nuffield Department of Clinical Neurosciences, University of Oxford, OX3 9DU Oxford, UK. Electronic address: adele.smart@ndcn.ox.ac.uk., Tisca C; Wellcome Centre for Integrative Neuroimaging, FMRIB, Nuffield Department of Clinical Neurosciences, University of Oxford, OX3 9DU Oxford, UK., Huszar IN; Wellcome Centre for Integrative Neuroimaging, FMRIB, Nuffield Department of Clinical Neurosciences, University of Oxford, OX3 9DU Oxford, UK., Kor D; Wellcome Centre for Integrative Neuroimaging, FMRIB, Nuffield Department of Clinical Neurosciences, University of Oxford, OX3 9DU Oxford, UK., Ansorge O; Neuropathology, Nuffield Department of Clinical Neurosciences, University of Oxford, OX3 9DU Oxford, UK., Tachrount M; Wellcome Centre for Integrative Neuroimaging, FMRIB, Nuffield Department of Clinical Neurosciences, University of Oxford, OX3 9DU Oxford, UK., Smart S; Wellcome Centre for Integrative Neuroimaging, FMRIB, Nuffield Department of Clinical Neurosciences, University of Oxford, OX3 9DU Oxford, UK., Lerch JP; Wellcome Centre for Integrative Neuroimaging, FMRIB, Nuffield Department of Clinical Neurosciences, University of Oxford, OX3 9DU Oxford, UK., Miller KL; Wellcome Centre for Integrative Neuroimaging, FMRIB, Nuffield Department of Clinical Neurosciences, University of Oxford, OX3 9DU Oxford, UK., Martins-Bach AB; Wellcome Centre for Integrative Neuroimaging, FMRIB, Nuffield Department of Clinical Neurosciences, University of Oxford, OX3 9DU Oxford, UK.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2023 Dec 15; Vol. 4 (4), pp. 102681. Date of Electronic Publication: 2023 Nov 09.
DOI:	10.1016/j.xpro.2023.102681
Abstrakt:	Combining histology and ex vivo MRI from the same mouse brain is a powerful way to study brain microstructure. Mouse brains prepared for ex vivo MRI are often kept in storage solution for months, potentially becoming brittle and showing reduced antigenicity. Here, we describe a protocol for mouse brain dissection, tissue processing, paraffin embedding, sectioning, and staining. We then detail registration of histology to ex vivo MRI data from the same sample and extraction of quantitative histological measurements. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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