Gelatin Carbon Dots Interaction with Nitrosyl Ruthenium Complex: Fluorescence Quenching and Chemiluminescence Mechanisms.

Autor: Astolfi BS; Instituto de Ciências Exatas e Naturais do Pontal, ICENP, Universidade Federal de Uberlândia, Rua Vinte, Tupã, Ituiutaba, MG, 1600, 38304-402, Brazil., Bessas NC; Instituto de Ciências Exatas e Naturais do Pontal, ICENP, Universidade Federal de Uberlândia, Rua Vinte, Tupã, Ituiutaba, MG, 1600, 38304-402, Brazil.; Instituto de Química, Universidade Federal de Uberlândia, Avenida João Naves de Ávila, Uberlândia, MG, 2121, 38400-902, Brazil., Graminha AE; Faculdade de Ciências Farmacêuticas de Ribeirão Preto, USP, Avenida do Café s/n, Vila Monte Alegre, Ribeirão Preto, SP, 14040-903, Brazil.; Instituto de Química, Universidade do Estado de São Paulo, Av. Prof. Francisco Degni, 55, Araraquara, São Paulo, 14800-900, Brazil., Becceneri AB; Faculdade de Ciências Farmacêuticas de Ribeirão Preto, USP, Avenida do Café s/n, Vila Monte Alegre, Ribeirão Preto, SP, 14040-903, Brazil., da Silva RS; Faculdade de Ciências Farmacêuticas de Ribeirão Preto, USP, Avenida do Café s/n, Vila Monte Alegre, Ribeirão Preto, SP, 14040-903, Brazil., de Lima RG; Instituto de Ciências Exatas e Naturais do Pontal, ICENP, Universidade Federal de Uberlândia, Rua Vinte, Tupã, Ituiutaba, MG, 1600, 38304-402, Brazil. renatagalvao@ufu.br.; Instituto de Química, Universidade Federal de Uberlândia, Avenida João Naves de Ávila, Uberlândia, MG, 2121, 38400-902, Brazil. renatagalvao@ufu.br.
Jazyk: angličtina
Zdroj: Journal of fluorescence [J Fluoresc] 2023 Nov 10. Date of Electronic Publication: 2023 Nov 10.
DOI: 10.1007/s10895-023-03490-y
Abstrakt: Carbon dots (CDs) exhibit luminescence, biocompatibility, and higher water solubility. This material has been developed for biological applications, specifically in bioimaging. In this work, the gelatin carbon dots (CD g ) was obtained from commercial gelatin using a hydrothermal method in domestic microwave, and the suppression fluorescent mechanism were enhanced by the addition of the [Ru II (bdq)(NO)(tpy)] 3+ (Rubdq-NO + ) complex ion. After purification through a dialysis bag, the resulting CDs (CD g ) exhibit fluorescent emission at 400 nm and maintained fluorescence stability in an aqueous solution (pH = 7) for 30 days under 5 ◦ C. Fluorescence quenching studies revealed an electrostatic interaction between the negative charge from CD g (δ =  - 20 mV) and the positively charged nitrosyl (NO + ) ligand of the ruthenium complex (Rubdq-NO + ), resulting in quenching of the CD g fluorescence due to the inner filter effects (IFE). The chemiluminescence reaction of CD g and Rubdq-NO-CD g in presence of norepinephrine (NOR) were evaluated. NOR in PBS are liable to undergo spontaneous oxidation to quinone form (NOR-quinone). CD g are believed interact with NOR-quinone and an electron transfer occur obtained CD g + accompanied to green emission fluorescence (520 nm). While for Rubdq-NO-CD g in presence of NOR, the green emission occurs accompanied by NO 0 release using DAF-2 probe.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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