Impact of Surface Ligand on the Biocompatibility of InP/ZnS Quantum Dots with Platelets.

Autor: Gil HM; Department of Imaging Chemistry and Biology, School of Biomedical Engineering and Imaging Sciences, King's College London, London, SE1 7EH, UK.; Centre for Biomedicine, Hull York Medical School, University of Hull, Hull, HU6 7RX, UK., Booth Z; Centre for Biomedicine, Hull York Medical School, University of Hull, Hull, HU6 7RX, UK., Price TW; Department of Imaging Chemistry and Biology, School of Biomedical Engineering and Imaging Sciences, King's College London, London, SE1 7EH, UK., Lee J; Centre for Biomedicine, University of Hull, Hull , HU6 7RX, UK., Naylor-Adamson L; Centre for Biomedicine, Hull York Medical School, University of Hull, Hull, HU6 7RX, UK., Avery M; Centre for Biomedicine, University of Hull, Hull , HU6 7RX, UK., Muravitskaya A; Department of Physics and Mathematics, University of Hull, Hull , HU6 7RX, UK., Hondow N; School of Chemical and Process Engineering, University of Leeds, Leeds, LS2 9JT, UK., Allsup D; Centre for Biomedicine, Hull York Medical School, University of Hull, Hull, HU6 7RX, UK., Schneider JE; School of Medicine, University of Leeds, Leeds, LS2 9JT, UK., Naseem K; Leeds Institute of Cardiovascular and Metabolic Medicine, University of Leeds, Leeds, LS2 9JT, UK., Adawi AM; Department of Physics and Mathematics, University of Hull, Hull , HU6 7RX, UK., Bouillard JG; Department of Physics and Mathematics, University of Hull, Hull , HU6 7RX, UK., Chamberlain TW; Institute of Process Research and Development School of Chemistry, University of Leeds, Leeds, LS2 9JT, UK., Calaminus SDJ; Centre for Biomedicine, Hull York Medical School, University of Hull, Hull, HU6 7RX, UK., Stasiuk GJ; Department of Imaging Chemistry and Biology, School of Biomedical Engineering and Imaging Sciences, King's College London, London, SE1 7EH, UK.
Jazyk: angličtina
Zdroj: Small (Weinheim an der Bergstrasse, Germany) [Small] 2024 Mar; Vol. 20 (12), pp. e2304881. Date of Electronic Publication: 2023 Nov 09.
DOI: 10.1002/smll.202304881
Abstrakt: InP/ZnS quantum dots (QDs) have received a large focus in recent years as a safer alternative to heavy metal-based QDs. Given their intrinsic fluorescent imaging capabilities, these QDs can be potentially relevant for in vivo platelet imaging. The InP/ZnS QDs are synthesized and their biocompatibility investigated through the use of different phase transfer agents. Analysis of platelet function indicates that platelet-QD interaction can occur at all concentrations and for all QD permutations tested. However, as the QD concentration increases, platelet aggregation is induced by QDs alone independent of natural platelet agonists. This study helps to define a range of concentrations and coatings (thioglycolic acid and penicillamine) that are biocompatible with platelet function. With this information, the platelet-QD interaction can be identified using multiple methods. Fluorescent lifetime imaging microscopy (FLIM) and confocal studies have shown QDs localize on the surface of the platelet toward the center while showing evidence of energy transfer within the QD population. It is believed that these findings are an important stepping point for the development of fluorescent probes for platelet imaging.
(© 2023 The Authors. Small published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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