Antimicrobial photodynamic therapy against Lactobacillus casei using curcumin, nano-curcumin, or erythrosine and a dental LED curing device.

Autor: Ahrari F; Dental Research Center, School of Dentistry, Mashhad University of Medical Sciences, Vakilabad Blvd, Mashhad, Iran. Farzaneh.Ahrari@Gmail.com., Mazhari F; Dental Material Research Center, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran., Ghazvini K; Department of Microbiology, Antimicrobial Resistance Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran., Fekrazad R; Laser Research Center in Medical Sciences, AJA University of Medical Sciences, Tehran, Iran., Menbari S; Department of Medical Laboratory Sciences, School of Paramedical, Kurdistan University of Medical Sciences, Sanandaj, Iran.; Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran., Nazifi M; Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran. M.nazifi95@gmail.com.
Jazyk: angličtina
Zdroj: Lasers in medical science [Lasers Med Sci] 2023 Nov 10; Vol. 38 (1), pp. 260. Date of Electronic Publication: 2023 Nov 10.
DOI: 10.1007/s10103-023-03914-y
Abstrakt: This study aimed to investigate the photodynamic effects of curcumin, nanomicelle curcumin, and erythrosine on Lactobacillus casei (L. casei). Various concentrations of curcumin (1.5 g/L, 3 g/L), nano-curcumin (3 g/L), and erythrosine (100 µM/L, 250 µM/L) were tested either alone or combined with light irradiation (PDT effect) against L. casei in planktonic and biofilm cultures. The light was emitted from a light-emitting diode (LED) with a central wavelength of 450 nm. A 0.12% chlorhexidine digluconate (CHX) solution served as the positive control, and a solution containing neither photosensitizer nor light was the negative control group. The number of viable microorganisms was determined using serial dilution. There was a significant difference in the viability of L. casei in both planktonic and biofilm forms (P < 0.05). In the planktonic culture, the antibacterial effects of CHX and PDT groups with curcumin 3 g/L and erythrosine 250 µM/L were significantly greater than the other groups (P < 0.05). For L. casei biofilms, the greatest toxic effects were observed in CHX and PDT groups with curcumin 3 g/L, erythrosine 250 µmol/L, erythrosine 100 µmol/L, and nanomicelle curcumin 3 g/L, with a significant difference to other groups (P < 0.05). The antibacterial effects of all photosensitizers (except erythrosine 250 µmol/L at planktonic culture) enhanced significantly when combined with light irradiation (P < 0.05). PDT with curcumin 3 g/L or erythrosine 250 µmol/L produced comparable results to CHX against L. casei at both planktonic and biofilm cultures. Alternatively, PDT with erythrosine 100 µmol/L or nanomicelle curcumin 3 g/L could be suggested to kill L. casei biofilms.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.)
Databáze: MEDLINE
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