Optogenetic stimulation of vagal nerves for enhanced glucose-stimulated insulin secretion and β cell proliferation.

Autor: Kawana Y; Department of Metabolism and Diabetes, Tohoku University Graduate School of Medicine, Sendai, Japan., Imai J; Department of Metabolism and Diabetes, Tohoku University Graduate School of Medicine, Sendai, Japan. imai@med.tohoku.ac.jp., Morizawa YM; Super-network Brain Physiology, Tohoku University Graduate School of Life Sciences, Sendai, Japan., Ikoma Y; Super-network Brain Physiology, Tohoku University Graduate School of Life Sciences, Sendai, Japan., Kohata M; Department of Metabolism and Diabetes, Tohoku University Graduate School of Medicine, Sendai, Japan., Komamura H; Department of Metabolism and Diabetes, Tohoku University Graduate School of Medicine, Sendai, Japan., Sato T; Department of Metabolism and Diabetes, Tohoku University Graduate School of Medicine, Sendai, Japan., Izumi T; Department of Metabolism and Diabetes, Tohoku University Graduate School of Medicine, Sendai, Japan., Yamamoto J; Department of Metabolism and Diabetes, Tohoku University Graduate School of Medicine, Sendai, Japan., Endo A; Department of Metabolism and Diabetes, Tohoku University Graduate School of Medicine, Sendai, Japan., Sugawara H; Department of Metabolism and Diabetes, Tohoku University Graduate School of Medicine, Sendai, Japan., Kubo H; Department of Metabolism and Diabetes, Tohoku University Graduate School of Medicine, Sendai, Japan., Hosaka S, Munakata Y; Department of Metabolism and Diabetes, Tohoku University Graduate School of Medicine, Sendai, Japan., Asai Y; Department of Metabolism and Diabetes, Tohoku University Graduate School of Medicine, Sendai, Japan., Kodama S; Department of Metabolism and Diabetes, Tohoku University Graduate School of Medicine, Sendai, Japan., Takahashi K; Department of Metabolism and Diabetes, Tohoku University Graduate School of Medicine, Sendai, Japan., Kaneko K; Department of Metabolism and Diabetes, Tohoku University Graduate School of Medicine, Sendai, Japan., Sawada S; Division of Metabolism and Diabetes, Faculty of Medicine, Tohoku Medical and Pharmaceutical University, Sendai, Japan., Yamada T; Department of Molecular Endocrinology and Metabolism, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan., Ito A; Department of Neurosurgery, Tohoku University Graduate School of Medicine, Sendai, Japan., Niizuma K; Department of Neurosurgery, Tohoku University Graduate School of Medicine, Sendai, Japan.; Department of Neurosurgical Engineering and Translational Neuroscience, Tohoku University Graduate School of Medicine, Sendai, Japan.; Department of Neurosurgical Engineering and Translational Neuroscience, Graduate School of Biomedical Engineering, Tohoku University, Sendai, Japan., Tominaga T; Department of Neurosurgery, Tohoku University Graduate School of Medicine, Sendai, Japan., Yamanaka A; Department of Neuroscience II, Research Institute of Environmental Medicine, Nagoya University, Nagoya, Japan., Matsui K; Super-network Brain Physiology, Tohoku University Graduate School of Life Sciences, Sendai, Japan., Katagiri H; Department of Metabolism and Diabetes, Tohoku University Graduate School of Medicine, Sendai, Japan.
Jazyk: angličtina
Zdroj: Nature biomedical engineering [Nat Biomed Eng] 2024 Jul; Vol. 8 (7), pp. 808-822. Date of Electronic Publication: 2023 Nov 09.
DOI: 10.1038/s41551-023-01113-2
Abstrakt: The enhancement of insulin secretion and of the proliferation of pancreatic β cells are promising therapeutic options for diabetes. Signals from the vagal nerve regulate both processes, yet the effectiveness of stimulating the nerve is unclear, owing to a lack of techniques for doing it so selectively and prolongedly. Here we report two optogenetic methods for vagal-nerve stimulation that led to enhanced glucose-stimulated insulin secretion and to β cell proliferation in mice expressing choline acetyltransferase-channelrhodopsin 2. One method involves subdiaphragmatic implantation of an optical fibre for the photostimulation of cholinergic neurons expressing a blue-light-sensitive opsin. The other method, which suppressed streptozotocin-induced hyperglycaemia in the mice, involves the selective activation of vagal fibres by placing blue-light-emitting lanthanide microparticles in the pancreatic ducts of opsin-expressing mice, followed by near-infrared illumination. The two methods show that signals from the vagal nerve, especially from nerve fibres innervating the pancreas, are sufficient to regulate insulin secretion and β cell proliferation.
(© 2023. The Author(s).)
Databáze: MEDLINE
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