Evidence of abnormality in glutathione metabolism in the airways of preterm born children with a history of bronchopulmonary dysplasia.

Autor: Course CW; Department of Child Health, School of Medicine, Cardiff University, Heath Park, Cardiff, CF14 4XN, UK., Lewis PA; Faculty of Life Sciences, University of Bristol, Bristol, UK., Kotecha SJ; Department of Child Health, School of Medicine, Cardiff University, Heath Park, Cardiff, CF14 4XN, UK., Cousins M; Department of Child Health, School of Medicine, Cardiff University, Heath Park, Cardiff, CF14 4XN, UK.; Department of Paediatrics, Cardiff and Vale University Health Board, Cardiff, UK., Hart K; Department of Paediatrics, Cardiff and Vale University Health Board, Cardiff, UK., Heesom KJ; Faculty of Life Sciences, University of Bristol, Bristol, UK., Watkins WJ; Department of Child Health, School of Medicine, Cardiff University, Heath Park, Cardiff, CF14 4XN, UK., Kotecha S; Department of Child Health, School of Medicine, Cardiff University, Heath Park, Cardiff, CF14 4XN, UK. kotechas@cardiff.ac.uk.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Nov 09; Vol. 13 (1), pp. 19465. Date of Electronic Publication: 2023 Nov 09.
DOI: 10.1038/s41598-023-46499-w
Abstrakt: Preterm-born children are at risk of long-term pulmonary deficits, including those who developed bronchopulmonary dysplasia (BPD) in infancy, however the underlying mechanisms remain poorly understood. We characterised the exhaled breath condensate (EBC) metabolome from preterm-born children, both with and without BPD. Following spirometry, EBC from children aged 7-12 years, from the Respiratory Health Outcomes in Neonates study, were analysed using Time-of-Flight Mass Spectrometry. Metabolite Set Enrichment Analysis (MSEA) linked significantly altered metabolites to biological processes. Linear regression models examined relationships between metabolites of interest and participant demographics. EBC was analysed from 214 children, 144 were born preterm, including 34 with BPD. 235 metabolites were detected, with 38 above the detection limit in every sample. Alanine and pyroglutamic acid were significantly reduced in the BPD group when compared to preterm controls. MSEA demonstrated a reduction in glutathione metabolism. Reduced quantities of alanine, ornithine and urea in the BPD group were linked with alteration of the urea cycle. Linear regression revealed significant associations with BPD when other characteristics were considered, but not with current lung function parameters. In this exploratory study of the airway metabolome, preterm-born children with a history of BPD had changes consistent with reduced antioxidant mechanisms suggesting oxidative stress.
(© 2023. The Author(s).)
Databáze: MEDLINE
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