Fabrication and evaluation of anticancer potential of diosgenin incorporated chitosan-silver nanoparticles; in vitro, in silico and in vivo studies.

Autor: Zaib S; Department of Basic and Applied Chemistry, Faculty of Science and Technology, University of Central Punjab, Lahore 54590, Pakistan. Electronic address: sumera.zaib@ucp.edu.pk., Shah HS; Institute of Pharmaceutical Sciences, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan. Electronic address: hamid.saeed@uvas.edu.pk., Khan I; Manchester Institute of Biotechnology, The University of Manchester, 131 Princess Street, Manchester M1 7DN, United Kingdom. Electronic address: kimtiaz@hotmail.co.uk., Jawad Z; Ladywillingdon Hospital, King Edward Medical University, Lahore, Pakistan., Sarfraz M; College of Pharmacy, Al Ain University, Al Ain 64141, United Arab Emirates., Riaz H; Department of Basic and Applied Chemistry, Faculty of Science and Technology, University of Central Punjab, Lahore 54590, Pakistan., Asjad HMM; Department of Pharmaceutical Sciences, Faculty of Biomedical Sciences and Engineering, Pak-Austria Fachhochschule: Institute of Applied Sciences and Technology, Mang, Khanpur Road, Haripur, KPK, Pakistan., Ishtiaq M; Leads College of Pharmacy, Lahore LEADS University, Lahore, Pakistan., Ogaly HA; Chemistry Department, College of Science, King Khalid University, Abha 61421, Saudi Arabia., Othman G; Biology Department, College of Science, King Khalid University, Abha 61421, Saudi Arabia., Ahmed DAEM; Biology Department, College of Science, King Khalid University, Abha 61421, Saudi Arabia.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Jan; Vol. 254 (Pt 3), pp. 127975. Date of Electronic Publication: 2023 Nov 08.
DOI: 10.1016/j.ijbiomac.2023.127975
Abstrakt: The discovery of effective therapeutic approaches with minimum side effects and their tendency to completely eradicate the disease is the main challenge in the history of cancer treatment. Fenugreek (FGK) seeds are a rich source of phytochemicals, especially Diosgenin (DGN), which shows outstanding anticancer activities. In the present study, chitosan-silver nanoparticles (ChAgNPs) containing Diosgenin (DGN-ChAgNPs) were synthesized and evaluated for their anticancer activity against breast cancer cell line (MCF-7). For the physical characterization, the hydrodynamic diameter and zeta potential of DGN-ChAgNPs were determined to be 160.4 ± 12 nm and +37.19 ± 5.02 mV, respectively. Transmission electron microscopy (TEM) showed that nanoparticles shape was mostly round with smooth edges. Moreover, DGN was efficiently entrapped in nanoformulation with good entrapment efficacy (EE) of ~88 ± 4 %. The in vitro anti-proliferative activity of DGN-ChAgNPs was performed by sulforhodamine B (SRB) assay with promising inhibitory concentration of 6.902 ± 2.79 μg/mL. DAPI staining, comet assay and flow cytometry were performed to validate the anticancer potential of DGN-ChAgNPs both qualitatively and quantitatively. The percentage of survival rate and tumor reduction weight was evaluated in vivo in different groups of mice. Cisplatin was used as a standard anticancer drug. The DGN-ChAgNPs (12.5 mg/kg) treated group revealed higher percentage of survival rate and tumor reduction weight as compared to pure DGN treated group. These findings suggest that DGN-ChAgNPs could be developed as potential treatment therapy for breast cancer.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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