Inhibition of FSP1 impairs early embryo developmental competence in pigs.

Autor: Wang YQ; Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun, China., Qu HX; Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun, China., Dong YW; Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun, China., Qi JJ; Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun, China., Wei HK; Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun, China., Sun H; Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun, China., Jiang H; Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun, China., Zhang JB; Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun, China., Sun BX; Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun, China. Electronic address: Sunpathing@vip.163.com., Liang S; Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun, China. Electronic address: liangshuang85@jlu.edu.cn.
Jazyk: angličtina
Zdroj: Theriogenology [Theriogenology] 2024 Jan 15; Vol. 214, pp. 257-265. Date of Electronic Publication: 2023 Oct 26.
DOI: 10.1016/j.theriogenology.2023.10.025
Abstrakt: Ferroptosis suppressor protein 1 (FSP1) is a glutathione-independent ferroptosis inhibitory factor. FSP1 has been found to play a crucial role in the regulation of mitochondrial function and ferroptosis. However, its function in porcine early embryonic development remains unknown. In the present research, we found that FSP1 was expressed at different stages during porcine early embryo development. Compared with the control condition, inhibition of FSP1 reduced the cleavage rate at 24 h and 48 h and the blastocyst rate at 144 h. In addition, inhibiting FSP1 reduced the blastocyst diameter, total cell number, and proliferation capacity. Further analysis showed that inhibition of FSP1 significantly increased the levels of ferrous ions (Fe 2+ ) and MDA but not GPX4. We also found that inhibition of FSP1 significantly decreased mitochondrial membrane potential and ATP levels, which in turn caused excessive accumulation of ROS and decreased the levels of GSH and the activity of the intracellular antioxidant enzymes SOD and CAT in embryos. In conclusion, FSP1, an important regulator, participates in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and further leads to oxidative stress due to mitochondrial dysfunction, ultimately affecting the developmental competence and impairing the quality of porcine early embryos.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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