Synthesis of novel oxytetracycline-molecularly imprinted polymer using a semi-covalent chemical imprinting approach for magnetic dispersive solid phase extraction of tetracyclines in water and milk samples.

Autor: Effting LM; Laboratório de Desenvolvimento de Métodos Analíticos (LADEMA), Departamento de Química, Universidade Estadual de Londrina (UEL), 86057-970 Londrina, PR, Brazil., Urbano A; Departamento de Física, Universidade Estadual de Londrina (UEL), 86057-970 Londrina, PR, Brazil., do Lago AC; Laboratório de Análises de Toxicantes e Fármacos, Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas, 37130-000 Alfenas, MG, Brazil., de Figueiredo EC; Laboratório de Análises de Toxicantes e Fármacos, Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas, 37130-000 Alfenas, MG, Brazil., Tarley CRT; Laboratório de Desenvolvimento de Métodos Analíticos (LADEMA), Departamento de Química, Universidade Estadual de Londrina (UEL), 86057-970 Londrina, PR, Brazil; Instituto Nacional de Ciência e Tecnologia de Bioanalítica - (INCTbio), Universidade Estadual de Campinas (UNICAMP), 13083-970 Campinas, SP, Brazil. Electronic address: tarley@uel.br.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2024 Mar 30; Vol. 437 (Pt 2), pp. 137854. Date of Electronic Publication: 2023 Nov 07.
DOI: 10.1016/j.foodchem.2023.137854
Abstrakt: In the present study, a highly efficient analytical method based on preconcentration of tetracyclines (TCs) in water and milk samples using a magnetic molecularly imprinted polymer (Fe 3 O 4 /SiO 2 /MIP) synthesized by means of a novel semi-covalent synthesis for TCs and determination by HPLC-DAD was proposed. Fe 3 O 4 /SiO 2 /MIP showed specific superficial area of 216.0 m 2 /g, superior to Fe 3 O 4 /SiO 2 /NIP (non-imprinted material) (103.6 m 2 /g). Using the Fe 3 O 4 /SiO 2 /MIP, the magnetic dispersive solid phase extraction (MDSPE) for four TCs (chlortetracycline, doxycycline, oxytetracycline, and tetracycline) was based on the preconcentration of 40.0 mL of sample (pH 4.0) vortex-assisted with 10 mg of Fe 3 O 4 /SiO 2 /MIP during 60 s. The method presented a limit of detection (LOD) in the range 0.26-0.60 µg/L, and preconcentration factor in the range 52.3-87.4-fold for the TCs. The method was applied to determine TCs in samples of lake water and cow milk with recovery results ranging from 96.0 to 102.0 %.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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