A new nanobiotic: synthesis and characterization of an albumin nanoparticle with intrinsic antibiotic activity.
| Autor: | Sheikh Hosseini M; Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran., Moosavi-Nejad Z; Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran., Mohammadi P; Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Iranian journal of microbiology [Iran J Microbiol] 2023 Oct; Vol. 15 (5), pp. 697-704. |
| DOI: | 10.18502/ijm.v15i5.13875 |
| Abstrakt: | Background and Objectives: With entering the "post-antibiotic era", antibiotic resistance is one of the most important problems in food security, health and medicine. Invention of nanoparticles with intrinsic antimicrobial activity has been provided a new tool to combat the problem, including some metal nanoparticles. But protein nanoparticles have been often used as nano-carrier for antibiotic drugs, not for their own antibiotic activity. In this article we have fabricated a very small BSA-NP without any chemical modification on BSA molecules showing antibacterial activity. Materials and Methods: Bovine serum albumin nanoparticle (BSA-NP) was synthesized using botton-up approach, by dissolution of BSA in urea-containing Tris buffer for 60 min at 60°C. Then, the BSA solution was dialyzed against distilled water in order to nanoparticle formation. The resulted BSA-NP has been characterized by dynamic light scattering (DLS), field emission surface electron microscopy (FESEM), SDS-PAGE, Fourier transform infrared spectroscopy (FTIR) and UV-spectrophotometery. Minimum inhibitory concentration (MIC) method was used for evaluation of antibacterial activity of BSA-NP against Staphylococcus aureus and Pseudomonas aeruginosa. Results: The results obtained by DLS technique indicated that BSA molecules were self-assembled into small aggregates with a hydrodynamic diameter of 23.23 ± 2.1 nm. With a small polydispersity index (PDI=0.522), the nanoparticles had good spherical uniformity. The nanoparticles made from a single type of protein molecule (BSA) and have a relatively transparent appearance. The BSA-NPs caused a decrease in cell growth of both P. aeruginosa and S. aureus. Moreover, they had a bacteriostatic effect on P. aeruginosa (MIC=112×10 -5 μM). Conclusion: In this study, using a green synthesis method, we succeeded in synthesizing a very small uniform BSA nanoparticles without any chemical modification on BSA molecules. It also has bacteriostatic properties against P. aeruginosa. Therefore, it is hypothesized that our BSA-NPs may be effective as a new approach to combat antibiotic resistance. (Copyright © 2023 The Authors. Published by Tehran University of Medical Sciences.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|