Genome-wide transcription landscape of citric acid producing Aspergillus niger in response to glucose gradient.

Autor:	Zheng X; College of Biotechnology, Tianjin University of Science & Technology, Tianjin, China.; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.; National Technology Innovation Center of Synthetic Biology, Tianjin, China.; University of Chinese Academy of Sciences, Beijing, China., Du P; College of Biotechnology, Tianjin University of Science & Technology, Tianjin, China.; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China., Gao K; College of Biotechnology, Tianjin University of Science & Technology, Tianjin, China.; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China., Du Y; College of Biotechnology, Tianjin University of Science & Technology, Tianjin, China.; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China., Cairns TC; Chair of Applied and Molecular Microbiology, Institute of Biotechnology, Technische Universität Berlin, Berlin, Germany., Ni X; College of Biotechnology, Tianjin University of Science & Technology, Tianjin, China.; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.; National Technology Innovation Center of Synthetic Biology, Tianjin, China., Chen M; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.; School of Biotechnology, East China University of Science and Technology, Shanghai, China., Zhao W; Shan Dong Fuyang Biological Technology Co., Ltd., Dezhou, China., Ma X; College of Biotechnology, Tianjin University of Science & Technology, Tianjin, China., Yang H; College of Biotechnology, Tianjin University of Science & Technology, Tianjin, China., Zheng P; College of Biotechnology, Tianjin University of Science & Technology, Tianjin, China.; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.; National Technology Innovation Center of Synthetic Biology, Tianjin, China.; University of Chinese Academy of Sciences, Beijing, China., Sun J; College of Biotechnology, Tianjin University of Science & Technology, Tianjin, China.; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.; National Technology Innovation Center of Synthetic Biology, Tianjin, China.; University of Chinese Academy of Sciences, Beijing, China.
Jazyk:	angličtina
Zdroj:	Frontiers in bioengineering and biotechnology [Front Bioeng Biotechnol] 2023 Oct 24; Vol. 11, pp. 1282314. Date of Electronic Publication: 2023 Oct 24 (Print Publication: 2023).
DOI:	10.3389/fbioe.2023.1282314
Abstrakt:	Aspergillus niger is the main industrial workhorse for global citric acid production. This fungus has complex sensing and signaling pathways to respond to environmental nutrient fluctuations. As the preferred primary carbon source, glucose also acts as a critical signal to trigger intracellular bioprocesses. Currently, however, there is still a knowledge gap in systems-level understanding of metabolic and cellular responses to this vital carbon source. In this study, we determined genome-wide transcriptional changes of citric acid-producing Aspergillus niger in response to external glucose gradient. It demonstrated that external glucose fluctuation led to transcriptional reprogramming of many genes encoding proteins involved in fundamental cellular process, including ribosomal biogenesis, carbon transport and catabolism, glucose sensing and signaling. The major glucose catabolism repressor creA maintained a stable expression independent of external glucose, while creB and creD showed significant downregulation and upregulation by the glucose increase. Notably, several high-affinity glucose transporters encoding genes, including mstA , were greatly upregulated when glucose was depleted, while the expression of low-affinity glucose transporter mstC was glucose-independent, which showed clear concordance with their protein levels detected by in situ fluorescence labeling assay. In addition, we also observed that the citric acid exporter cexA was observed to be transcriptionally regulated by glucose availability, which was correlated with extracellular citric acid secretion. These discoveries not only deepen our understanding of the transcriptional regulation of glucose but also shed new light on the adaptive evolutionary mechanism of citric acid production of A. niger . Competing Interests: Author WZ was employed by Shan Dong Fuyang Biological Technology Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Zheng, Du, Gao, Du, Cairns, Ni, Chen, Zhao, Ma, Yang, Zheng and Sun.)
Databáze:	MEDLINE
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