A clinical proteomics study of exhaled breath condensate and biomarkers for pulmonary embolism.

Autor:	Gade IL; Department of Hematology and Clinical Cancer Research Center, Aalborg University Hospital, 9000 Aalborg, Denmark., Riddersholm SJ; Department of Cardiology, Aalborg University Hospital, 9000 Aalborg, Denmark., Stilling-Vinther T; Emergency Care Center, Aalborg University Hospital, 9000 Aalborg, Denmark., Brøndum RF; Center for Clinical Data Science, Aalborg University and Aalborg University Hospital, 9260 Gistrup, Denmark., Bennike TB; Department of Health Science and Technology, Aalborg University, Aalborg, Denmark., Honoré B; Department of Clinical Medicine, Aalborg University, 9000 Aalborg, Denmark.; Department of Biomedicine, Aarhus University, 8000 Aarhus, Denmark.
Jazyk:	angličtina
Zdroj:	Journal of breath research [J Breath Res] 2023 Nov 17; Vol. 18 (1). Date of Electronic Publication: 2023 Nov 17.
DOI:	10.1088/1752-7163/ad0aaa
Abstrakt:	Pulmonary embolism (PE) can be a diagnostic challenge. Current diagnostic markers for PE are unspecific and new diagnostic tools are needed. The air we exhale is a possible new source for biomarkers which can be tapped into by analysing the exhaled breath condensate (EBC). We analysed the EBC from patients with PE and controls to investigate if the EBC is a useful source for new diagnostic biomarkers of PE. We collected and analysed EBC samples from patients with suspected PE and controls matched on age and sex. Patients in whom PE was ruled out after diagnostic work-up were included in the control group to increase the sensitivity and generalizability of the identified markers. EBC samples were collected using an RTube™. The protein composition of the EBCs were analysed using data dependent label-free quantitative nano liquid chromatography-tandem mass spectrometry. EBC samples from 28 patients with confirmed PE, and 49 controls were analysed. A total of 928 EBC proteins were identified in the 77 EBC samples. As expected, a low protein concentration was determined which resulted in many proteins with unmeasurable levels in several samples. The levels of HSPA5, PEBP1 and SFTPA2 were higher and levels of POF1B, EPPK1, PSMA4, ALDOA, and CFL1 were lower in PE compared with controls. In conclusion, the human EBC contained a variety of endogenous proteins and may be a source for new diagnostic markers of PE and other diseases. (Creative Commons Attribution license.)
Databáze:	MEDLINE
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