Bioavailable iron titrations reveal oceanic Synechococcus ecotypes optimized for different iron availabilities.

Autor: Gilbert NE; Department of Microbiology, The University of Tennessee, Knoxville, TN, 37996, USA., LeCleir GR; Department of Microbiology, The University of Tennessee, Knoxville, TN, 37996, USA., Strzepek RF; Institute for Marine and Antarctic Studies, University of Tasmania, Hobart, TAS, 7004, Australia.; Australian Antarctic Program Partnership (AAPP), Institute for Marine and Antarctic Studies, University of Tasmania, Hobart, TAS, 7004, Australia., Ellwood MJ; Research School of Earth Sciences, Australian National University, Canberra, ACT, Australia., Twining BS; Bigelow Laboratory for Ocean Sciences, East Boothbay, ME, USA., Roux S; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Pennacchio C; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Boyd PW; Institute for Marine and Antarctic Studies, University of Tasmania, Hobart, TAS, 7004, Australia., Wilhelm SW; Department of Microbiology, The University of Tennessee, Knoxville, TN, 37996, USA. wilhelm@utk.edu.
Jazyk: angličtina
Zdroj: ISME communications [ISME Commun] 2022 Jul 01; Vol. 2 (1), pp. 54. Date of Electronic Publication: 2022 Jul 01.
DOI: 10.1038/s43705-022-00132-5
Abstrakt: The trace metal iron (Fe) controls the diversity and activity of phytoplankton across the surface oceans, a paradigm established through decades of in situ and mesocosm experimental studies. Despite widespread Fe-limitation within high-nutrient, low chlorophyll (HNLC) waters, significant contributions of the cyanobacterium Synechococcus to the phytoplankton stock can be found. Correlations among differing strains of Synechococcus across different Fe-regimes have suggested the existence of Fe-adapted ecotypes. However, experimental evidence of high- versus low-Fe adapted strains of Synechococcus is lacking, and so we investigated the transcriptional responses of microbial communities inhabiting the HNLC, sub-Antarctic region of the Southern Ocean during the Spring of 2018. Analysis of metatranscriptomes generated from on-deck incubation experiments reflecting a gradient of Fe-availabilities reveal transcriptomic signatures indicative of co-occurring Synechococcus ecotypes adapted to differing Fe-regimes. Functional analyses comparing low-Fe and high-Fe conditions point to various Fe-acquisition mechanisms that may allow persistence of low-Fe adapted Synechococcus under Fe-limitation. Comparison of in situ surface conditions to the Fe-titrations indicate ecological relevance of these mechanisms as well as persistence of both putative ecotypes within this region. This Fe-titration approach, combined with transcriptomics, highlights the short-term responses of the in situ phytoplankton community to Fe-availability that are often overlooked by examining genomic content or bulk physiological responses alone. These findings expand our knowledge about how phytoplankton in HNLC Southern Ocean waters adapt and respond to changing Fe supply.
(© 2022. The Author(s).)
Databáze: MEDLINE
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