Characterization of Molecular Tweezer Binding on α-Synuclein with Native Top-Down Mass Spectrometry and Ion Mobility-Mass Spectrometry Reveals a Mechanism for Aggregation Inhibition.

Autor: Lantz C; Department of Chemistry and Biochemistry, University of California─Los Angeles, Los Angeles, California 90095, United States., Lopez J; Department of Chemistry and Biochemistry, University of California─Los Angeles, Los Angeles, California 90095, United States., Goring AK; Department of Chemistry and Biochemistry, University of California─Los Angeles, Los Angeles, California 90095, United States., Zenaidee MA; Department of Chemistry and Biochemistry, University of California─Los Angeles, Los Angeles, California 90095, United States.; Australian Proteome Analysis Facility, Macquarie University, Macquarie Park, New South Wales 2109, Australia., Biggs K; Department of Neurology and Brain Research Institute, David Geffen School of Medicine at UCLA, University of California─Los Angeles, Los Angeles, California 90095, United States., Whitelegge JP; The Pasarow Mass Spectrometry Laboratory, The Jane and Terry Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine at UCLA, University of California─Los Angeles, Los Angeles, California 90095, United States., Ogorzalek Loo RR; Department of Chemistry and Biochemistry, University of California─Los Angeles, Los Angeles, California 90095, United States., Klärner FG; Institute of Chemistry, University of Duisburg-Essen, Essen 45141, Germany., Schrader T; Institute of Chemistry, University of Duisburg-Essen, Essen 45141, Germany., Bitan G; Department of Neurology and Brain Research Institute, David Geffen School of Medicine at UCLA, University of California─Los Angeles, Los Angeles, California 90095, United States.; Molecular Biology Institute, University of California─Los Angeles, Los Angeles, California 90095, United States., Loo JA; Department of Chemistry and Biochemistry, University of California─Los Angeles, Los Angeles, California 90095, United States.; Molecular Biology Institute, University of California─Los Angeles, Los Angeles, California 90095, United States.; Department of Biological Chemistry, David Geffen School of Medicine at UCLA, University of California─Los Angeles, Los Angeles, California 90095, United States.
Jazyk: angličtina
Zdroj: Journal of the American Society for Mass Spectrometry [J Am Soc Mass Spectrom] 2023 Dec 06; Vol. 34 (12), pp. 2739-2747. Date of Electronic Publication: 2023 Nov 07.
DOI: 10.1021/jasms.3c00281
Abstrakt: Parkinson's disease, a neurodegenerative disease that affects 15 million people worldwide, is characterized by deposition of α-synuclein into Lewy Bodies in brain neurons. Although this disease is prevalent worldwide, a therapy or cure has yet to be found. Several small compounds have been reported to disrupt fibril formation. Among these compounds is a molecular tweezer known as CLR01 that targets lysine and arginine residues. This study aims to characterize how CLR01 interacts with various proteoforms of α-synuclein and how the structure of α-synuclein is subsequently altered. Native mass spectrometry (nMS) measurements of α-synuclein/CLR01 complexes reveal that multiple CLR01 molecules can bind to α-synuclein proteoforms such as α-synuclein phosphorylated at Ser-129 and α-synuclein bound with copper and manganese ions. The binding of one CLR01 molecule shifts the ability for α-synuclein to bind other ligands. Electron capture dissociation (ECD) with Fourier transform-ion cyclotron resonance (FT-ICR) top-down (TD) mass spectrometry of α-synuclein/CLR01 complexes pinpoints the locations of the modifications on each proteoform and reveals that CLR01 binds to the N-terminal region of α-synuclein. CLR01 binding compacts the gas-phase structure of α-synuclein, as shown by ion mobility-mass spectrometry (IM-MS). These data suggest that when multiple CLR01 molecules bind, the N-terminus of α-synuclein shifts toward a more compact state. This compaction suggests a mechanism for CLR01 halting the formation of oligomers and fibrils involved in many neurodegenerative diseases.
Databáze: MEDLINE
Externí odkaz: