UV-C irradiation as an effective tool for sterilization of porcine chimeric VP1-PCV2bCap recombinant vaccine.

Autor: Vrablikova A; Department of Pharmacology and Toxicology, Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic. alena.vrablikova@vri.cz., Fojtikova M; Department of Pharmacology and Toxicology, Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic., Hezova R; Department of Pharmacology and Toxicology, Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic., Simeckova P; Department of Pharmacology and Toxicology, Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic., Brezani V; Department of Pharmacology and Toxicology, Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic., Strakova N; Department of Pharmacology and Toxicology, Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic., Fraiberk M; Faculty of Science, Charles University, Albertov 6, 128 00, Prague, Czech Republic., Kotoucek J; Department of Pharmacology and Toxicology, Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic., Masek J; Department of Pharmacology and Toxicology, Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic., Psikal I; Department of Pharmacology and Toxicology, Veterinary Research Institute, Hudcova 70, 621 00, Brno, Czech Republic.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Nov 07; Vol. 13 (1), pp. 19337. Date of Electronic Publication: 2023 Nov 07.
DOI: 10.1038/s41598-023-46791-9
Abstrakt: Ultraviolet irradiation is an effective method of virus and bacteria inactivation. The dose of UV-C light necessary for baculovirus inactivation by measurement of fluorescent GFP protein produced by baculovirus expression system after the irradiation of baculovirus culture in doses ranging from 3.5 to 42 J/m 2 was determined. At a dose of 36.8 J/m 2 , only 0.5% of GFP-expressing cells were detected by flow cytometry and confocal microscopy. The stability of purified VP1-PCV2bCap protein produced by baculovirus expression system was analyzed after the irradiation at doses ranging from 3.5 to 19.3 J/m 2 . Up to the dose of 11 J/m 2 , no significant effect of UV-C light on the stability of VP1-PCV2bCap was detected. We observed a dose-dependent increase in VP1-PCV2bCap-specific immune response in BALB/c mice immunized by recombinant protein sterilized by irradiation in dose 11 J/m 2 with no significant difference between vaccines sterilized by UV-C light and filtration. A substantial difference in the production of VP1-PCV2bCap specific IgG was observed in piglets immunized with VP1-PCV2bCap sterilized by UV-C in comparison with protein sterilized by filtration in combination with the inactivation of baculovirus by binary ethylenimine. UV-C irradiation represents an effective method for vaccine sterilization, where commonly used methods of sterilization are not possible.
(© 2023. The Author(s).)
Databáze: MEDLINE
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