Biocompatible Rhamnolipid Self-Assemblies with pH-Responsive Antimicrobial Activity.

Autor: Kadakia P; Department of Chemistry, University of Fribourg, Chemin du Musée 9, Fribourg, 1700, Switzerland., Valentin JDP; Department of Chemistry, University of Fribourg, Chemin du Musée 9, Fribourg, 1700, Switzerland., Hong L; Department of Chemistry, University of Fribourg, Chemin du Musée 9, Fribourg, 1700, Switzerland., Watts S; Department of Chemistry, University of Fribourg, Chemin du Musée 9, Fribourg, 1700, Switzerland., Hameed OA; Department of Chemistry, University of Fribourg, Chemin du Musée 9, Fribourg, 1700, Switzerland.; Anatomy Unit, Department of Oncology, Microbiology and Immunology, Faculty of Science and Medicine, University of Fribourg, Fribourg, 1700, Switzerland., Walch M; Anatomy Unit, Department of Oncology, Microbiology and Immunology, Faculty of Science and Medicine, University of Fribourg, Fribourg, 1700, Switzerland., Salentinig S; Department of Chemistry, University of Fribourg, Chemin du Musée 9, Fribourg, 1700, Switzerland.
Jazyk: angličtina
Zdroj: Advanced healthcare materials [Adv Healthc Mater] 2024 Feb; Vol. 13 (4), pp. e2302596. Date of Electronic Publication: 2023 Nov 23.
DOI: 10.1002/adhm.202302596
Abstrakt: There is an urgent need for alternative antimicrobial materials due to the growing challenge of bacteria becoming resistant to conventional antibiotics. This study demonstrates the creation of a biocompatible pH-switchable antimicrobial material by combining bacteria-derived rhamnolipids (RL) and food-grade glycerol monooleate (GMO). The integration of RL into dispersed GMO particles, with an inverse-type liquid crystalline cubic structure in the core, leads to colloidally stable supramolecular materials. The composition and pH-triggered structural transformations are studied with small-angle X-ray scattering, cryogenic transmission electron microscopy, and dynamic light scattering. The composition-structure-activity relationship is analyzed and optimized to target bacteria at acidic pH values of acute wounds. The new RL/GMO dispersions reduce Staphylococcus aureus (S. aureus) populations by 7-log after 24 h of treatment with 64 µg mL -1 of RL and prevent biofilm formation at pH = 5.0, but have no activity at pH = 7.0. Additionally, the system is active against methicillin-resistant S. aureus (MRSA) with minimum inhibitory concentration of 128 µg mL -1 at pH 5.0. No activity is found against several Gram-negative bacteria at pH 5.0 and 7.0. The results provide a fundamental understanding of lipid self-assembly and the design of lipid-based biomaterials, which can further guide the development of alternative bio-based solutions to combat bacteria.
(© 2023 Wiley-VCH GmbH.)
Databáze: MEDLINE
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