| Autor: |
Varma VP; Laboratory of Vaccine Immunology, National Institute of Animal Biotechnology, Hyderabad 500032, India.; Graduate Studies, Manipal Academy of Higher Education, Manipal 576104, Karnataka, India., Bankala R; Laboratory of Vaccine Immunology, National Institute of Animal Biotechnology, Hyderabad 500032, India., Kumar A; Laboratory of Vaccine Immunology, National Institute of Animal Biotechnology, Hyderabad 500032, India.; Regional Centre for Biotechnology, Faridabad, India., Gawai S; Laboratory of Vaccine Immunology, National Institute of Animal Biotechnology, Hyderabad 500032, India., Faisal SM; Laboratory of Vaccine Immunology, National Institute of Animal Biotechnology, Hyderabad 500032, India.; Regional Centre for Biotechnology, Faridabad, India. |
| Abstrakt: |
Leptospirosis is a worldwide zoonosis caused by pathogenic Leptospira spp. having more than 300 serovars. These serovars can infect a variety of hosts, some being asymptomatic carriers and others showing varied symptoms of mild to severe infection. Since lipopolysaccharide (LPS) is the major antigen which defines serovar specificity, this different course of infection may be attributed to a differential innate response against this antigen. Previous studies have shown that Leptospira LPS is less endotoxic. However, it is unclear whether there is a difference in the ability of LPS isolated from different serovars to modulate the innate response. In this study, we purified LPS from three widely prevalent pathogenic serovars, i.e. Icterohaemorrhagiae strain RGA, Pomona, Hardjo, and from non-pathogenic L. biflexa serovar semeranga strain Potac 1 collectively termed as L-LPS and tested their ability to modulate innate response in macrophages from both resistant (mice) and susceptible (human and bovine) hosts. L-LPS induced differential response being more proinflammatory in mouse and less proinflammatory in human and bovine macrophages but overall less immunostimulatory than E. coli LPS (E-LPS). Irrespective of serovar, this response was TLR2-dependent in humans, whereas TLR4-dependent/CD14-independent in mouse using MyD88 adapter and signalling through P38 and ERK-dependent MAP kinase pathway. L-LPS-activated macrophages were able to phagocytose Leptospira and this effect was significantly higher or more pronounced when the macrophages were stimulated with L-LPS from the corresponding serovar. L-LPS activated both canonical and non-canonical inflammasome, producing IL-1β without inducing pyroptosis. Further, L-LPS induced both TNF-mediated early and NO-mediated late apoptosis. Altogether, these results indicate that L-LPS induces a differential innate response that is quite distinct from that induced by E-LPS and may be attributed to the structural differences and its atypical nature. |
