NLISA versus enzyme-linked immunosorbent assay: Nanozyme-linked immunosorbent array based on platinum sub-nanocluster nanozyme for α-fetoprotein detection.

Autor: Zhang H; The Fifth Hospital of Xiamen, Xiamen, China., Wang Q; The Second Affiliated Hospital of Fujian University of Traditional Chinese Medicine, Fujian-Macao Science and Technology Cooperation Base of Traditional Chinese Medicine-Oriented Chronic Disease Prevention and Treatment, Innovation and Transformation Center, Fujian University of Traditional Chinese Medicine, Fuzhou, China.; College of Life Sciences, Fujian Normal University, Fuzhou, China., Cai F; College of Life Sciences, Fujian Normal University, Fuzhou, China., Huang C; The Fifth Hospital of Xiamen, Xiamen, China., Wang Y; The Fifth Hospital of Xiamen, Xiamen, China., Zhang J; The Fifth Hospital of Xiamen, Xiamen, China., Huang J; The Fifth Hospital of Xiamen, Xiamen, China.
Jazyk: angličtina
Zdroj: Luminescence : the journal of biological and chemical luminescence [Luminescence] 2024 Jan; Vol. 39 (1), pp. e4620. Date of Electronic Publication: 2023 Nov 07.
DOI: 10.1002/bio.4620
Abstrakt: Rapid and accurate identification of tumor metabolic markers is important for early tumor diagnosis and individualized treatment. Here, a stable monodisperse sub-nanometer platinum (Pt) material was developed as a highly efficient nanozyme with a specific activity of peroxidase as high as 20.86 U mg -1 through the growth of in situ domain-limited Pt quantum dots via the polymer polyvinylpyrrolidone. Further, the synthesis of large quantities of Pt-loaded SiO 2 (Pt-SiO 2 ) was determined by silylation reaction and used for naked eye colorimetric testing of human alpha-fetoprotein (AFP). In particular, the immunization incubation process occurred in preprepared microplates. A nanozyme-based immunomodel was constructed in the presence of the target AFP, and a chromogenic reaction occurred with exogenous hydrogen peroxide and the chromogenic substrate tetramethylbenzidine. On optimization of experimental conditions, the dynamic working response range for AFP was found to be 0.05-20 ng mL -1 , with a limit of detection of 38.7 pg mL -1 . This work provides a new strategy to design efficient nanozyme-based enzyme-linked immunochromatographic platforms to meet the practical use of replacing natural enzymes.
(© 2023 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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