Structure-activity analysis suggests an olfactory function for the unique antennal delta glutathione transferase of Apis mellifera.

Autor: Schwartz M; CSGA, Flavour Perception: Molecular Mechanisms (Flavours), Université de Bourgogne, INRAE, CNRS, Institut Agro, Dijon, France., Boichot V; CSGA, Flavour Perception: Molecular Mechanisms (Flavours), Université de Bourgogne, INRAE, CNRS, Institut Agro, Dijon, France., Muradova M; CSGA, Flavour Perception: Molecular Mechanisms (Flavours), Université de Bourgogne, INRAE, CNRS, Institut Agro, Dijon, France.; International Research Center 'Biotechnologies of the Third Millennium', Faculty of Biotechnologies (BioTech), ITMO University, Saint-Petersburg, Russia., Fournier P; Independent Researcher, Charlotte, NC, USA., Senet P; Laboratoire Interdisciplinaire Carnot de Bourgogne, UMR 6303 CNRS, Université de Bourgogne Franche-Comté, Dijon, France., Nicolai A; Laboratoire Interdisciplinaire Carnot de Bourgogne, UMR 6303 CNRS, Université de Bourgogne Franche-Comté, Dijon, France., Canon F; CSGA, Flavour Perception: Molecular Mechanisms (Flavours), Université de Bourgogne, INRAE, CNRS, Institut Agro, Dijon, France., Lirussi F; Plateforme PACE, Laboratoire de Pharmacologie-Toxicologie, Bioinformatique & Big Data Au Service de La Santé 2B2S, UFR Santé, Université de Franche-Comté, INSERM U1231, Centre Hospitalier Universitaire, Besançon, France., Ladeira R; Plateforme PACE, Laboratoire de Pharmacologie-Toxicologie, Bioinformatique & Big Data Au Service de La Santé 2B2S, UFR Santé, Université de Franche-Comté, INSERM U1231, Centre Hospitalier Universitaire, Besançon, France., Maibeche M; Institut d'Ecologie et des Sciences de l'Environnement de Paris, Sorbonne Université, INRAE, CNRS, IRD, UPEC, Paris, France., Chertemps T; Institut d'Ecologie et des Sciences de l'Environnement de Paris, Sorbonne Université, INRAE, CNRS, IRD, UPEC, Paris, France., Aubert E; Université de Lorraine, CNRS, CRM2, Nancy, France., Didierjean C; Université de Lorraine, CNRS, CRM2, Nancy, France., Neiers F; CSGA, Flavour Perception: Molecular Mechanisms (Flavours), Université de Bourgogne, INRAE, CNRS, Institut Agro, Dijon, France.
Jazyk: angličtina
Zdroj: FEBS letters [FEBS Lett] 2023 Dec; Vol. 597 (24), pp. 3038-3048. Date of Electronic Publication: 2023 Nov 07.
DOI: 10.1002/1873-3468.14770
Abstrakt: Glutathione transferases (GST) are detoxification enzymes that conjugate glutathione to a wide array of molecules. In the honey bee Apis mellifera, AmGSTD1 is the sole member of the delta class of GSTs, with expression in antennae. Here, we structurally and biochemically characterized AmGSTD1 to elucidate its function. We showed that AmGSTD1 can efficiently catalyse the glutathione conjugation of classical GST substrates. Additionally, AmGSTD1 exhibits binding properties with a range of odorant compounds. AmGSTD1 has a peculiar interface with a structural motif we propose to call 'sulfur sandwich'. This motif consists of a cysteine disulfide bridge sandwiched between the sulfur atoms of two methionine residues and is stabilized by CH…S hydrogen bonds and S…S sigma-hole interactions. Thermal stability studies confirmed that this motif is important for AmGSTD1 stability and, thus, could facilitate its functions in olfaction.
(© 2023 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.)
Databáze: MEDLINE
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