The impact of saliva collection methods on measured salivary biomarker levels.

Autor: Al Habobe H; Research Group Innovative Testing in Life Sciences and Chemistry, Research Centre for Healthy and Sustainable Living, University of Applied Sciences Utrecht, The Netherlands; Institute for Risk Assessment Sciences, Faculty of Veterinary Medicines, Utrecht University, The Netherlands; Dept of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Amsterdam, The Netherlands. Electronic address: hanan.alhabobe@hu.nl., Haverkort EB; Research Group Innovations in Preventive Care, Utrecht University of Applied Sciences, The Netherlands., Nazmi K; Dept of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Amsterdam, The Netherlands., Van Splunter AP; Dept of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Amsterdam, The Netherlands., Pieters RHH; Research Group Innovative Testing in Life Sciences and Chemistry, Research Centre for Healthy and Sustainable Living, University of Applied Sciences Utrecht, The Netherlands; Institute for Risk Assessment Sciences, Faculty of Veterinary Medicines, Utrecht University, The Netherlands., Bikker FJ; Dept of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Amsterdam, The Netherlands.
Jazyk: angličtina
Zdroj: Clinica chimica acta; international journal of clinical chemistry [Clin Chim Acta] 2024 Jan 01; Vol. 552, pp. 117628. Date of Electronic Publication: 2023 Nov 04.
DOI: 10.1016/j.cca.2023.117628
Abstrakt: Saliva diagnostics have become increasingly popular due to their non-invasive nature and patient-friendly collection process. Various collection methods are available, yet these are not always well standardized for either quantitative or qualitative analysis. In line, the objective of this study was to evaluate if measured levels of various biomarkers in the saliva of healthy individuals were affected by three distinct saliva collection methods: 1) unstimulated saliva, 2) chew stimulated saliva, and 3) oral rinse. Saliva samples from 30 healthy individuals were obtained by the three collection methods. Then, the levels of various salivary biomarkers such as proteins and ions were determined. It was found that levels of various biomarkers obtained from unstimulated saliva were comparable to those in chew stimulated saliva. The levels of potassium, sodium, and amylase activity differed significantly among the three collection methods. Levels of all biomarkers measured using the oral rinse method significantly differed from those obtained from unstimulated and chew-stimulated saliva. In conclusion, both unstimulated and chew-stimulated saliva provided comparable levels for a diverse group of biomarkers. However, the results obtained from the oral rinse method significantly differed from those of unstimulated and chew-stimulated saliva, due to the diluted nature of the saliva extract.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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