Quantitative Determination of Antibacterial Activity During Bacterial Coculture.

Autor:	Alcoforado Diniz J; Division of Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee, UK., Earl C; Division of Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee, UK., Hernandez RE; Division of Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee, UK., Hollmann B; Division of Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee, UK., Coulthurst SJ; Division of Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee, UK. s.j.coulthurst@dundee.ac.uk.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2715, pp. 593-600.
DOI:	10.1007/978-1-0716-3445-5_37
Abstrakt:	Antibacterial activity assays are an important tool in the assessment of the ability of one bacterium to kill or inhibit the growth of another, for example, during the study of the Type VI secretion system (T6SS) and the antibacterial toxins it secretes. The method we describe here can detect the ability of a bacterial strain to kill or inhibit other bacterial cells in a contact-dependent manner when cocultured on an agar surface. It is particularly useful since it enumerates the recovery of viable target cells and thus enables quantification of the antibacterial activity. We provide a detailed description of how to measure the T6SS-dependent antibacterial activity of a bacterium such as Serratia marcescens against a competitor prokaryotic organism, Escherichia coli, and describe possible variations in the method to allow adaptation to other attacker and target organisms. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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