Protein-Protein Interactions: Co-immunoprecipitation.

Autor: Lin JS; Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan., Ali J; Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.; Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, National Chung-Hsing University and Academia Sinica, Taipei, Taiwan.; Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, Taiwan., Lai EM; Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan. emlai@gate.sinica.edu.tw.; Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, National Chung-Hsing University and Academia Sinica, Taipei, Taiwan. emlai@gate.sinica.edu.tw.; Biotechnology Center, National Chung-Hsing University, Taichung, Taiwan. emlai@gate.sinica.edu.tw.
Jazyk: angličtina
Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2715, pp. 273-283.
DOI: 10.1007/978-1-0716-3445-5_18
Abstrakt: Proteins often do not function as single substances but rather as team players in a dynamic network. Growing evidences show that protein-protein interactions are crucial in many biological processes in living cells. Genetic (such as yeast two hybrid, Y2H) and biochemical (such as co-immunoprecipitation, co-IP) methods are the commonly used methods to identify the interacting proteins. Immunoprecipitation (IP), a method using a target protein-specific antibody in conjunction with Protein A/G affinity beads, is a powerful tool to identify the molecules interacting with specific proteins. Therefore, co-IP is considered to be one of the standard methods to identify and/or confirm the occurrence of the protein-protein interaction events in vivo. The co-IP experiments can identify proteins via direct or indirect interactions or in a protein complex. Here, we use two different co-Ip protocols as an example to describe the principle, procedure, and experimental problems of co-IP. First, we show the interaction of two Agrobacterium type VI secretion system (T6SS) sheath components TssB and TssC 41 , and secondly, we show the protocol we used for determining the interaction of an epitope-tagged T6SS effector, Tde1 expressed in Agrobacterium with endogenously expressing adaptor/chaperone protein Tap1.
(© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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