Large-Scale Development of Antibacterial Scaffolds: Gentamicin Sulfate-Loaded Biodegradable Nanofibers for Gastrointestinal Applications.

Autor:	Klicova M; Department of Nonwovens and Nanofibrous Materials, Faculty of Textile Engineering, Technical University of Liberec, Studentska 2, Liberec 461 17, Czech Republic., Mullerova S; Department of Nonwovens and Nanofibrous Materials, Faculty of Textile Engineering, Technical University of Liberec, Studentska 2, Liberec 461 17, Czech Republic., Rosendorf J; Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Alej Svobody 1655/76, Plzen 323 00, Czech Republic., Klapstova A; Department of Nonwovens and Nanofibrous Materials, Faculty of Textile Engineering, Technical University of Liberec, Studentska 2, Liberec 461 17, Czech Republic., Jirkovec R; Department of Nonwovens and Nanofibrous Materials, Faculty of Textile Engineering, Technical University of Liberec, Studentska 2, Liberec 461 17, Czech Republic., Erben J; Department of Nonwovens and Nanofibrous Materials, Faculty of Textile Engineering, Technical University of Liberec, Studentska 2, Liberec 461 17, Czech Republic., Petrzilkova M; Faculty of Mechatronics, Informatics and Interdisciplinary Studies, Institute of New Technologies and Applied Informatics, Studentska 1402/2, Liberec 461 17, Czech Republic., Raabová H; Faculty of Pharmacy, The Department of Analytical Chemistry, Charles University, Akademika Heyrovskeho 1203, Hradec Kralove 500 05, Czech Republic., Šatínský D; Faculty of Pharmacy, The Department of Analytical Chemistry, Charles University, Akademika Heyrovskeho 1203, Hradec Kralove 500 05, Czech Republic., Melicherikova J; The Institute for Nanomaterials, Advanced Technologies and Innovation, Technical University of Liberec, Bendlova 7, Liberec 460 01, Czech Republic., Palek R; Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Alej Svobody 1655/76, Plzen 323 00, Czech Republic., Liska V; Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Alej Svobody 1655/76, Plzen 323 00, Czech Republic., Horakova J; Department of Nonwovens and Nanofibrous Materials, Faculty of Textile Engineering, Technical University of Liberec, Studentska 2, Liberec 461 17, Czech Republic.
Jazyk:	angličtina
Zdroj:	ACS omega [ACS Omega] 2023 Oct 17; Vol. 8 (43), pp. 40823-40835. Date of Electronic Publication: 2023 Oct 17 (Print Publication: 2023).
DOI:	10.1021/acsomega.3c05924
Abstrakt:	The ever-increasing demands of modern medicine drive the development of novel drug delivery materials. In particular, nanofibers are promising for such materials due to their favorable properties. However, most development is still carried out through laboratory techniques that do not allow extensive and reproducible characterization of materials, which slows medical research. In this work, we focus on the large-scale fabrication and testing of specific antibacterial nanofibrous materials to prevent the postoperative complications associated with the occurrence of bacterial infection. Poly-ε-caprolactone with gentamicin sulfate (antibiotic) in different concentrations was electrospun via a needleless device. The amount of antibiotics was proven by elemental analysis, UV spectrophotometry, and HPLC. The cytocompatibility of the materials was verified in vitro according to ISO 10993-5. The cell adhesion and proliferation were assessed after 2, 7, 14, and 21 days using the CCK-8 metabolic assay, fluorescence, and scanning electron microscopy. The tested nanofiber materials supported cell growth. Antibacterial tests were performed to confirm the release of gentamicin sulfate, and its antibacterial properties were proven toward Staphylococcus gallinarum and Escherichia coli bacteria. The effect of ethylene oxide sterilization was also studied. The sterilized nanofibrous layers are cytocompatible while antibacterial and therefore suitable for medical applications. Competing Interests: The authors declare no competing financial interest. (© 2023 The Authors. Published by American Chemical Society.)
Databáze:	MEDLINE
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