Mass cytometry analysis reveals altered immune profiles in patients with coronary artery disease.

Autor: Kott KA; Cardiothoracic and Vascular Health Kolling Institute of Medical Research Sydney NSW Australia.; Department of Cardiology, Royal North Shore Hospital Northern Sydney Local Health District Sydney NSW Australia.; Northern Clinical School, Faculty of Medicine and Health University of Sydney Sydney NSW Australia., Chan AS; School of Mathematics and Statistics University of Sydney Sydney NSW Australia.; Charles Perkins Centre University of Sydney Sydney NSW Australia., Vernon ST; Cardiothoracic and Vascular Health Kolling Institute of Medical Research Sydney NSW Australia.; Department of Cardiology, Royal North Shore Hospital Northern Sydney Local Health District Sydney NSW Australia.; Northern Clinical School, Faculty of Medicine and Health University of Sydney Sydney NSW Australia., Hansen T; Cardiothoracic and Vascular Health Kolling Institute of Medical Research Sydney NSW Australia., Kim T; School of Mathematics and Statistics University of Sydney Sydney NSW Australia.; Charles Perkins Centre University of Sydney Sydney NSW Australia., de Dreu M; School of Medical Sciences, Faculty of Medicine and Health University of Sydney Sydney NSW Australia., Gunasegaran B; Charles Perkins Centre University of Sydney Sydney NSW Australia.; School of Medical Sciences, Faculty of Medicine and Health University of Sydney Sydney NSW Australia., Murphy AJ; Baker Heart and Diabetes Institute Melbourne VIC Australia., Patrick E; School of Mathematics and Statistics University of Sydney Sydney NSW Australia.; Charles Perkins Centre University of Sydney Sydney NSW Australia., Psaltis PJ; Monash Cardiovascular Research Centre Clayton VIC Australia., Grieve SM; Charles Perkins Centre University of Sydney Sydney NSW Australia.; School of Medical Sciences, Faculty of Medicine and Health University of Sydney Sydney NSW Australia.; Department of Radiology Royal Prince Alfred Hospital Sydney NSW Australia.; Imaging and Phenotyping Laboratory, Charles Perkins Centre, Faculty of Medicine and Health University of Sydney Sydney NSW Australia., Yang JY; School of Mathematics and Statistics University of Sydney Sydney NSW Australia.; Charles Perkins Centre University of Sydney Sydney NSW Australia., Fazekas de St Groth B; Charles Perkins Centre University of Sydney Sydney NSW Australia.; School of Medical Sciences, Faculty of Medicine and Health University of Sydney Sydney NSW Australia.; Ramaciotti Facility for Human Systems Biology University of Sydney Sydney NSW Australia., McGuire HM; Charles Perkins Centre University of Sydney Sydney NSW Australia.; School of Medical Sciences, Faculty of Medicine and Health University of Sydney Sydney NSW Australia.; Ramaciotti Facility for Human Systems Biology University of Sydney Sydney NSW Australia., Figtree GA; Cardiothoracic and Vascular Health Kolling Institute of Medical Research Sydney NSW Australia.; Department of Cardiology, Royal North Shore Hospital Northern Sydney Local Health District Sydney NSW Australia.; Northern Clinical School, Faculty of Medicine and Health University of Sydney Sydney NSW Australia.; Charles Perkins Centre University of Sydney Sydney NSW Australia.
Jazyk: angličtina
Zdroj: Clinical & translational immunology [Clin Transl Immunology] 2023 Nov 02; Vol. 12 (11), pp. e1462. Date of Electronic Publication: 2023 Nov 02 (Print Publication: 2023).
DOI: 10.1002/cti2.1462
Abstrakt: Objective: The importance of inflammation in atherosclerosis is well accepted, but the role of the adaptive immune system is not yet fully understood. To further explore this, we assessed the circulating immune cell profile of patients with coronary artery disease (CAD) to identify discriminatory features by mass cytometry.
Methods: Mass cytometry was performed on patient samples from the BioHEART-CT study, gated to detect 82 distinct cell subsets. CT coronary angiograms were analysed to categorise patients as having CAD (CAD + ) or having normal coronary arteries (CAD - ).
Results: The discovery cohort included 117 patients (mean age 61 ± 12 years, 49% female); 79 patients (68%) were CAD + . Mass cytometry identified changes in 15 T-cell subsets, with higher numbers of proliferating, highly differentiated and cytotoxic cells and decreases in naïve T cells. Five T-regulatory subsets were related to an age and gender-independent increase in the odds of CAD incidence when expressing CCR2 (OR 1.12), CCR4 (OR 1.08), CD38 and CD45RO (OR 1.13), HLA-DR (OR 1.06) and Ki67 (OR 1.22). Markers of proliferation and differentiation were also increased within B cells, while plasmacytoid dendritic cells were decreased. This combination of changes was assessed using SVM models in discovery and validation cohorts (area under the curve = 0.74 for both), confirming the robust nature of the immune signature detected.
Conclusion: We identified differences within immune subpopulations of CAD + patients which are indicative of a systemic immune response to coronary atherosclerosis. This immune signature needs further study via incorporation into risk scoring tools for the precision diagnosis of CAD.
Competing Interests: The authors declare no conflict of interest.
(© 2023 The Authors. Clinical & Translational Immunology published by John Wiley & Sons Australia, Ltd on behalf of Australian and New Zealand Society for Immunology, Inc.)
Databáze: MEDLINE
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