Dynamic Micropatterning Reveals Substrate-Dependent Differences in the Geometric Control of Cell Polarization and Migration.

Autor: Isomursu A; Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, 20520, Finland., Alanko J; Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, 20520, Finland., Hernández-Pérez S; Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, 20520, Finland.; Institute of Biomedicine and MediCity Research Laboratories, University of Turku, Turku, 20014, Finland.; Department of Life Technologies, University of Turku, Turku, 20520, Finland., Saukkonen K; Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, 20520, Finland., Saari M; Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, 20520, Finland., Mattila PK; Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, 20520, Finland.; Institute of Biomedicine and MediCity Research Laboratories, University of Turku, Turku, 20014, Finland.; Department of Life Technologies, University of Turku, Turku, 20520, Finland., Ivaska J; Turku Bioscience Centre, University of Turku and Åbo Akademi University, Turku, 20520, Finland.; Department of Life Technologies, University of Turku, Turku, 20520, Finland.; InFLAMES Research Flagship Center, University of Turku, Turku, 20520, Finland.; Western Finnish Cancer Center (FICAN West), University of Turku, Turku, 20520, Finland.; Foundation for the Finnish Cancer Institute, Helsinki, 00014, Finland.
Jazyk: angličtina
Zdroj: Small methods [Small Methods] 2024 Jan; Vol. 8 (1), pp. e2300719. Date of Electronic Publication: 2023 Nov 05.
DOI: 10.1002/smtd.202300719
Abstrakt: Cells are highly dynamic and adopt variable shapes and sizes. These variations are biologically important but challenging to investigate in a spatiotemporally controlled manner. Micropatterning, confining cells on microfabricated substrates with defined geometries and molecular compositions, is a powerful tool for controlling cell shape and interactions. However, conventional binary micropatterns are static and fail to address dynamic changes in cell polarity, spreading, and migration. Here, a method for dynamic micropatterning is reported, where the non-adhesive surface surrounding adhesive micropatterns is rapidly converted to support specific cell-matrix interactions while allowing simultaneous imaging of the cells. The technique is based on ultraviolet photopatterning of biotinylated polyethylene glycol-grafted poly-L-lysine, and it is simple, inexpensive, and compatible with a wide range of streptavidin-conjugated ligands. Experiments using biotinylation-based dynamic micropatterns reveal that distinct extracellular matrix ligands and bivalent integrin-clustering antibodies support different degrees of front-rear polarity in human glioblastoma cells, which correlates to altered directionality and persistence upon release and migration on fibronectin. Unexpectedly, however, neither an asymmetric cell shape nor centrosome orientation can fully predict the future direction of migration. Taken together, biotinylation-based dynamic micropatterns allow easily accessible and highly customizable control over cell morphology and motility.
(© 2023 The Authors. Small Methods published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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