Comparison of molecular techniques for the detection of Toxoplasma gondii in raw bovine milk from small rural properties in Brazil.

Autor: Manzini S; Tropical Diseases and Image Diagnosis Department, Medical College, Sao Paulo State University (UNESP), Botucatu, Brazil. Electronic address: suzane.manzini@hotmail.com., Bertozzo TV; Tropical Diseases and Image Diagnosis Department, Medical College, Sao Paulo State University (UNESP), Botucatu, Brazil. Electronic address: thainabertozzo@gmail.com., Aires IN; Tropical Diseases and Image Diagnosis Department, Medical College, Sao Paulo State University (UNESP), Botucatu, Brazil. Electronic address: aires.isabella@gmail.com., Rodrigues NJL; Tropical Diseases and Image Diagnosis Department, Medical College, Sao Paulo State University (UNESP), Botucatu, Brazil. Electronic address: nassarah_lot@yahoo.com.br., Bertolini AB; Animal Production and Preventive Veterinary Medicine Department (FMVZ), Sao Paulo State University (UNESP), Botucatu, Brazil. Electronic address: ab.bertolini@unesp.br., Alexandrino M; Tropical Diseases and Image Diagnosis Department, Medical College, Sao Paulo State University (UNESP), Botucatu, Brazil. Electronic address: marcela.alexandrino@unesp.br., Steinle JS; Animal Production and Preventive Veterinary Medicine Department (FMVZ), Sao Paulo State University (UNESP), Botucatu, Brazil. Electronic address: jackieline.steinle@unesp.br., de Melo RPB; Department of Veterinary Medicine, Rural Federal University of Pernambuco (UFRPE), Recife, Brazil. Electronic address: renata.pimentel@ufrpe.br., Mota RA; Department of Veterinary Medicine, Rural Federal University of Pernambuco (UFRPE), Recife, Brazil. Electronic address: rinaldo.mota@hotmail.com., de Medeiros MIM; São Paulo State Agency for Agribusiness Technology (APTA/SAA), Bauru, São Paulo, Brazil. Electronic address: maria.medeiros@sp.gov.br., Richini-Pereira VB; Adolfo Lutz Institute, Bauru, São Paulo State, Brazil. Electronic address: virichini@yahoo.com.br., Curci VCLM; Biological Institute, Araçatuba, São Paulo State, Brazil. Electronic address: vera.curci@sp.gov.br., Lucheis SB; Tropical Diseases and Image Diagnosis Department, Medical College, Sao Paulo State University (UNESP), Botucatu, Brazil; Animal Production and Preventive Veterinary Medicine Department (FMVZ), Sao Paulo State University (UNESP), Botucatu, Brazil; São Paulo State Agency for Agribusiness Technology (APTA/SAA), Bauru, São Paulo, Brazil. Electronic address: simone.b.lucheis@unesp.br.
Jazyk: angličtina
Zdroj: International journal of food microbiology [Int J Food Microbiol] 2024 Jan 16; Vol. 409, pp. 110466. Date of Electronic Publication: 2023 Oct 31.
DOI: 10.1016/j.ijfoodmicro.2023.110466
Abstrakt: This study aimed to research Toxoplasma gondii DNA in 102 samples of raw bovine milk from expansion tanks, in small properties located in different cities of the Midwest region of São Paulo, Brazil. For this, polymerase chain reaction (PCR) was performed with the primers TOX4/TOX5 for cPCR (conventional PCR), TgNP1/TgNP2 gene for nested PCR and the Tg18s58F/Tg18s348R for nested PCR. It was possible to detect T. gondii DNA in 18 (17.65 %) milk samples from the 102 tanks, corresponding to 4.90 % for TOX4/TOX5 primers, 12.74 % for TgNP1/TgNP2 gene and 0.98 % for Tg18s58F/Tg18s348R gene. The results showed that the TgNP1 and TgNP2 genes were more efficient to detect T. gondii DNA, and also indicated the importance of raw bovine milk as a source of human infections caused by this protozoan, being a public health problem. It is important to continue studies involving T. gondii from bovine milk considering the need for proper pasteurization, and for better comprehension regarding the epidemiology of this protozoan.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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