Potential benefits of Malva sylvestris in dry-eye disease pathology in vitro based on antioxidant, wound-healing and anti-inflammatory properties.

Autor: Areesanan A; Translational Complementary Medicine, Department of Pharmaceutical Sciences, University of Basel, Campus Rosental - Mattenstrasse 22, CH-4058 Basel, Switzerland., Nicolay S; Translational Complementary Medicine, Department of Pharmaceutical Sciences, University of Basel, Campus Rosental - Mattenstrasse 22, CH-4058 Basel, Switzerland., Keller M; Division of Pharmaceutical Biology, University of Basel, Klingelbergstrasse 50, CH-4056 Basel, Switzerland., Zimmermann-Klemd AM; Translational Complementary Medicine, Department of Pharmaceutical Sciences, University of Basel, Campus Rosental - Mattenstrasse 22, CH-4058 Basel, Switzerland., Potterat O; Division of Pharmaceutical Biology, University of Basel, Klingelbergstrasse 50, CH-4056 Basel, Switzerland., Gründemann C; Translational Complementary Medicine, Department of Pharmaceutical Sciences, University of Basel, Campus Rosental - Mattenstrasse 22, CH-4058 Basel, Switzerland. Electronic address: carsten.gruendemann@unibas.ch.
Jazyk: angličtina
Zdroj: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie [Biomed Pharmacother] 2023 Dec; Vol. 168, pp. 115782. Date of Electronic Publication: 2023 Nov 03.
DOI: 10.1016/j.biopha.2023.115782
Abstrakt: Dry eye disease (DED) is a common chronic ocular surface disease. Available therapies are effective but often associated with side effects. This study investigates the potential of a Malva sylvestris L. flower extract and two defined preparations, a mucilage and a polyphenol rich fraction, on cells that are essential for the DED pathology. Furthermore, single compounds were isolated and characterised out of the polyphenol fraction. The M. sylvestris extract and its two fractions reduced reactive oxygen species (ROS) in an ultraviolet-induced model and promoted wound healing capacity of HCE-T cells, but only the polyphenol fraction and the flower extract exhibited significant radical scavenging activity. The flower extract and the polyphenol fraction inhibited cytokine secretion (IL-6, TNF-α, IL-8) from HCE-T cells and THP-1 cells. In contrast, the mucilage fraction led to an increase in mediator secretion. The NF-κB activity and calcium influx in THP-1 and Jurkat cells, respectively was decreased by treatment with the flower extract and the polyphenol fraction, whereas the mucilage fraction had no influence on these parameters. Moreover, the flower extract and the mucilage fraction at low concentration could stimulate meibomian gland cells' lipid accumulation. The isolated single compounds showed no effect on analysed parameters, except a coumarin derivative and malvin which showed ROS inhibition effects.
Competing Interests: Declaration of Competing Interest All other authors declare no conflict of interest. The funding sponsors had no role in the design, writing and publishing strategy of the study, as well as in collection, analysis, or interpretation of the data.
(Copyright © 2023 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
Databáze: MEDLINE
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