Diagnostic and commensal Staphylococcus pseudintermedius genomes reveal niche adaptation through parallel selection of defense mechanisms.

Autor: Sawhney SS; The Edison Family Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, MO, USA., Vargas RC; The Edison Family Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, MO, USA., Wallace MA; Department of Pathology and Immunology, Division of Laboratory and Genomic Medicine, Washington University School of Medicine, St. Louis, MO, USA., Muenks CE; Department of Pathology and Immunology, Division of Laboratory and Genomic Medicine, Washington University School of Medicine, St. Louis, MO, USA., Lubbers BV; Department of Clinical Sciences, Kansas State University, Manhattan, KS, USA., Fritz SA; Department of Pediatrics, Washington University School of Medicine, St. Louis, MO, USA., Burnham CD; Department of Pathology and Immunology, Division of Laboratory and Genomic Medicine, Washington University School of Medicine, St. Louis, MO, USA. cburnham@wustl.edu.; Department of Pediatrics, Washington University School of Medicine, St. Louis, MO, USA. cburnham@wustl.edu.; Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA. cburnham@wustl.edu.; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA. cburnham@wustl.edu., Dantas G; The Edison Family Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, MO, USA. dantas@wustl.edu.; Department of Pathology and Immunology, Division of Laboratory and Genomic Medicine, Washington University School of Medicine, St. Louis, MO, USA. dantas@wustl.edu.; Department of Pediatrics, Washington University School of Medicine, St. Louis, MO, USA. dantas@wustl.edu.; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, USA. dantas@wustl.edu.; Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO, USA. dantas@wustl.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Nov 03; Vol. 14 (1), pp. 7065. Date of Electronic Publication: 2023 Nov 03.
DOI: 10.1038/s41467-023-42694-5
Abstrakt: Staphylococcus pseudintermedius is historically understood as a prevalent commensal and pathogen of dogs, though modern clinical diagnostics reveal an expanded host-range that includes humans. It remains unclear whether differentiation across S. pseudintermedius populations is driven primarily by niche-type or host-species. We sequenced 501 diagnostic and commensal isolates from a hospital, veterinary diagnostic laboratory, and within households in the American Midwest, and performed a comparative genomics investigation contrasting human diagnostic, animal diagnostic, human colonizing, pet colonizing, and household-surface S. pseudintermedius isolates. Though indistinguishable by core and accessory gene architecture, diagnostic isolates harbor more encoded and phenotypic resistance, whereas colonizing and surface isolates harbor similar CRISPR defense systems likely reflective of common household phage exposures. Furthermore, household isolates that persist through anti-staphylococcal decolonization report elevated rates of base-changing mutations in - and parallel evolution of - defense genes, as well as reductions in oxacillin and trimethoprim-sulfamethoxazole susceptibility. Together we report parallel niche-specific bolstering of S. pseudintermedius defense mechanisms through gene acquisition or mutation.
(© 2023. The Author(s).)
Databáze: MEDLINE
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